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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MPHOSPH9 Recombinant monoclonal antibody (83397-5-RR)

    WB analysis using 83397-5-RR (same clone as 83397-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83397-5-RR (MPHOSPH9 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83397-5-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using MPHOSPH9 Recombinant monoclonal antibody (83397-5-RR)

    WB analysis using 83397-5-RR (same clone as 83397-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83397-5-RR (MPHOSPH9 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83397-5-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of A431 cells using MPHOSPH9 Recombinant monoclonal antibody (83397-5-RR)

    FC experiment of A431 using 83397-5-RR (same clone as 83397-5-PBS)

    1x10^6 A431 cells were intracellularly stained with 0.25 ug MPHOSPH9 Recombinant antibody (83397-5-RR, Clone:240182D6) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 83397-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83397-5-RR

    Affinity and Kinetic Characterization of 83397-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83397-5-RR against Human MPHOSPH9 were performed. The affinity constant is below 1 pM.

MPHOSPH9 Recombinant monoclonal antibody, PBS Only

MPHOSPH9 Uni-rAb® Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 83397-5-PBS
Clone No.240182D6

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), ELISA

Synonyms

240182D6, M-phase phosphoprotein 9, MPP 9, MPP9

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83397-5-PBS targets MPHOSPH9 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag22228

Product name: Recombinant human MPHOSPH9 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 5-350 aa of BC130440

Sequence: SLSSERNESVIHYPESTEPEIQQEMSTSQPDCNVDSCSVSSGYGTFCISELNLYKSKDPKEFMEHIDVPKGQYVAPAVPAESLVDGVKNENFYIQTPEECHVSLKEDVSISPGEFEHNFLGENKVSEVYSGKTNSNAITSWAQKLKQNQPKRAHVEDGGSRSKQGNEQSKKTPIEKSDFAAATHPRAFYLSKPDETPNAWMSDSGTGLTYWKLEEKDMHHSLPETLEKTFISLSSTDVSPNQSNTSNEMKLPSLKDIYYKKQRENKQLPERNLTSASNPNHPPEVLTLDPTLHMKPKQQISGIQPHGLPNALDDRISFSPDSVLEPSMSSPSDIDSFSQASNVTSQ

相同性解析による交差性が予測される生物種
Full Name M-phase phosphoprotein 9
Calculated molecular weight 1031 aa, 116 kDa
GenBank accession numberBC130440
Gene Symbol MPHOSPH9
Gene ID (NCBI) 10198
RRIDAB_3671049
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDQ99550
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

MPHOSPH9, also known as MPP9, is a protein phosphorylated during mitosis. MPHOSPH9 negatively regulates cilia formation by recruiting the CP110-CEP97 complex at the distal end of the mother centriole in ciliary cells. MPHOSPH9 is a centrosome component that localizes to the distal and proximal ends of two centrioles(PMID: 30375385).

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