• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Use Able AI chat for product recommendations

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of MCF-7 cells using MPHOSPH6 Polyclonal antibody (10695-1-AP)

    WB analysis of MCF-7 using 10695-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 10695-1-AP (MPHOSPH6 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using MPHOSPH6 Polyclonal antibody (10695-1-AP)

    WB analysis of MCF-7 using 10695-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 10695-1-AP (MPHOSPH6 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using MPHOSPH6 Polyclonal antibody (10695-1-AP)

    WB analysis of HeLa using 10695-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 10695-1-AP (MPHOSPH6 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using MPHOSPH6 Polyclonal antibody (10695-1-AP)

    WB analysis of HeLa using 10695-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 10695-1-AP (MPHOSPH6 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MDA-MB-453s cells using MPHOSPH6 Polyclonal antibody (10695-1-AP)

    WB analysis of MDA-MB-453s using 10695-1-AP

    MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 10695-1-AP (MPHOSPH6 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using MPHOSPH6 Polyclonal antibody (10695-1-AP)

    IP experiment of HeLa using 10695-1-AP

    IP result of anti-MPHOSPH6 (IP:10695-1-AP, 4ug; Detection:10695-1-AP 1:500) with HeLa cells lysate 3000 ug.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using MPHOSPH6 Polyclonal antibody (10695-1-AP)

    IHC staining of human breast cancer using 10695-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 10695-1-AP (MPHOSPH6 antibody) at dilution of 1:50 (under 10x lens).

MPHOSPH6 Polyclonal antibody

MPHOSPH6 Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 10695-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, ELISA

Synonyms

M phase phosphoprotein 6, MPHOSPH6, MPP, MPP 6, MPP6

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inMCF7 cells, HeLa cells, MCF-7 cells, MDA-MB-453s cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10695-1-AP targets MPHOSPH6 in WB, IP, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag1080

Product name: Recombinant human MPHOSPH6 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-160 aa of BC005242

Sequence: MAAERKTKLSKNLLRMKFMQRGLDSETKKQLEEEEKKIISEEHWYLDLPELKEKESFIIEEQSFLLCEDLLYGRMSFRGFNPEVEKLMLQMNAKHKAEEVEDETVELDVSDEEMARRYETLVGTIGKKFARKRDHANYEEDENGDITPIKAKKMFLKPQD

相同性解析による交差性が予測される生物種
Full Name M-phase phosphoprotein 6
Calculated molecular weight 19 kDa
Observed molecular weight 19 kDa
GenBank accession numberBC005242
Gene Symbol MPHOSPH6
Gene ID (NCBI) 10200
RRIDAB_2282161
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ99547
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IHC protocol for MPHOSPH6 antibody 10695-1-APDownload protocol
IP protocol for MPHOSPH6 antibody 10695-1-APDownload protocol
WB protocol for MPHOSPH6 antibody 10695-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Cell Prolif

The 5.8S pre-rRNA maturation factor, M-phase phosphoprotein 6, is a female fertility factor required for oocyte quality and meiosis.

Authors - Rui-Rui Peng
View Article
||
New chat Able
正在加载，请稍候...