Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, MCF-7 cells |
| Positive IF/ICC detected in | A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
32275-1-AP targets MPP10 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag37602 Product name: Recombinant human MPHOSPH10 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 391-654 aa of BC126389 Sequence: TAQKRPENSLLEETLHFDHAVRMAPVITEETTLQLEDIIKQRIRDQAWDDVVRKEKPKEDAYEYKKRLTLDHEKSKLSLAEIYEQEYIKLNQQKTAEEENPEHVEIQKMMDSLFLKLDALSNFHFIPKPPVPEIKVVSNLPAITMEEVAPVSVSDAALLAPEEIKEKNKAGDIKTAAEKTATDKKRERRKKKYQKRMKIKEKEKRRKLLEKSSVDQAGKYSKTVASEKLKQLTKTGKASFIKDEGKDKALKSSQAFFSKLQDQV 相同性解析による交差性が予測される生物種 |
| Full Name | M-phase phosphoprotein 10 (U3 small nucleolar ribonucleoprotein) |
| Calculated molecular weight | 681 aa, 79 kDa |
| Observed molecular weight | 105 kDa |
| GenBank accession number | BC126389 |
| Gene Symbol | MPHOSPH10 |
| Gene ID (NCBI) | 10199 |
| RRID | AB_3742563 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | O00566 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
MPP10, also known as M phase phosphoprotein 10, is a component of the U3 small nucleolar ribonucleoprotein (U3 snoRNP) complex. The protein localizes to the nucleolus during interphase and to the chromosomes during M phase. It plays a crucial role in the early cleavages during pre-18S ribosomal RNA processing.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for MPP10 antibody 32275-1-AP | Download protocol |
| WB protocol for MPP10 antibody 32275-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |