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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using MPEG1 Polyclonal antibody (23960-1-AP)

    WB analysis of HeLa using 23960-1-AP (same clone as 23960-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 23960-1-AP (MPEG1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23960-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using MPEG1 Polyclonal antibody (23960-1-AP)

    WB analysis using 23960-1-AP (same clone as 23960-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 23960-1-AP (MPEG1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23960-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using MPEG1 Polyclonal antibody (23960-1-AP)

    WB analysis of HeLa using 23960-1-AP (same clone as 23960-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 23960-1-AP (MPEG1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23960-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human rectal cancer tissue using MPEG1 Polyclonal antibody (23960-1-AP)

    IHC staining of human rectal cancer using 23960-1-AP (same clone as 23960-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 23960-1-AP (MPEG1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23960-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human rectal cancer tissue using MPEG1 Polyclonal antibody (23960-1-AP)

    IHC staining of human rectal cancer using 23960-1-AP (same clone as 23960-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 23960-1-AP (MPEG1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23960-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat spleen tissue using MPEG1 Polyclonal antibody (23960-1-AP)

    IHC staining of rat spleen using 23960-1-AP (same clone as 23960-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 23960-1-AP (MPEG1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23960-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat spleen tissue using MPEG1 Polyclonal antibody (23960-1-AP)

    IHC staining of rat spleen using 23960-1-AP (same clone as 23960-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 23960-1-AP (MPEG1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23960-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using MPEG1 Polyclonal antibody (23960-1-AP)

    IHC staining of rat brain using 23960-1-AP (same clone as 23960-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 23960-1-AP (MPEG1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23960-1-PBS in a different storage buffer formulation.

MPEG1 Polyclonal antibody, PBS Only

MPEG1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 23960-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

macrophage expressed 1, Macrophage gene 1 protein, Macrophage-expressed gene 1 protein, Macrophage-expressed gene 1 protein, processed form, Mpg 1

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

23960-1-PBS targets MPEG1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag21055

Product name: Recombinant human MPEG1 protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 25-370 aa of BC104997

Sequence: EMDEVGVQKCKNALKLPVLEVLPGGGWDNLRNVDMGRVMELTYSNCRTTEDGQYIIPDEIFTIPQKQSNLEMNSEILESWANYQSSTSYSINTELSLFSKVNGKFSTEFQRMKTLQVKDQAITTRVQVRNLVYTVKINPTLELSSGFRKELLDISDRLENNQTRMATYLAELLVLNYGTHVTTSVDAGAALIQEDHLRASFLQDSQSSRSAVTASAGLAFQNTVNFKFEENYTSQNVLTKSYLSNRTNSRVQSIGGVPFYPGITLQAWQQGITNHLVAIDRSGLPLHFFINPNMLPDLPGPLVKKVSKTVETAVKRYYTFNTYPGCTDLNSPNFNFQANTDDGSCE

相同性解析による交差性が予測される生物種
Full Name macrophage expressed 1
Calculated molecular weight 716 aa, 79 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC104997
Gene Symbol MPEG1
Gene ID (NCBI) 219972
RRIDAB_2879376
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ2M385
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

MPEG1 (macrophage-expressed gene 1), also known as perforin-2, is a pore-forming protein of the membrane attack complex/perforin (MACPF) superfamily. Predominantly expressed in macrophages and leukocytes, it is upregulated by proinflammatory signals such as TNF-α and LPS. MPEG1 localizes to phagosomes and lysosomes, where it breaches bacterial membranes to facilitate antimicrobial defense and antigen cross-presentation. (PMID: 26402460)

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