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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MOS Polyclonal antibody

MOS Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26454-1-AP

Synonyms

c-mos, MOS, MSV, oncogene MOS, Oocyte maturation factor mos, Proto oncogene c Mos

Validation Data Gallery

Applications:
  • WB analysis using 26454-1-AP

    WB analysis using 26454-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26454-1-AP (MOS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 26454-1-AP

    WB analysis of HeLa using 26454-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 26454-1-AP (MOS Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 26454-1-AP

    IHC staining of human liver using 26454-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 26454-1-AP (MOS Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human liver using 26454-1-AP

    IHC staining of human liver using 26454-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 26454-1-AP (MOS Antibody) at dilution of 1:200 (under 40x lens).

  • IF Staining of HeLa using 26454-1-AP

    IF Staining of HeLa using 26454-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using MOS antibody (26454-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inA549 cells, HeLa cells, HEK-293 cells, Jurkat cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

26454-1-AP targets MOS in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MOS fusion protein Ag24585 相同性解析による交差性が予測される生物種
Full Name v-mos Moloney murine sarcoma viral oncogene homolog
Calculated molecular weight 346 aa, 38 kDa
Observed molecular weight 34-38 kDa
GenBank accession numberBC069569
Gene symbol MOS
Gene ID (NCBI) 4342
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Mos is a germ cell-specific serine/threonine protein kinase, can induce oncogenic transformation of somatic cells by direct phosphorylation of MAP kinase/ERK kinase (MEK1), activating the mitogen-activated protein kinases ERK1 and ERK2.

Protocols

Product Specific Protocols
WB protocol for MOS antibody 26454-1-APDownload protocol
IHC protocol for MOS antibody 26454-1-APDownload protocol
IF protocol for MOS antibody 26454-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols