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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using MORF4L2 Polyclonal antibody (14710-1-AP)

    WB analysis of HeLa using 14710-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 14710-1-AP (MORF4L2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using MORF4L2 Polyclonal antibody (14710-1-AP)

    WB analysis using 14710-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14710-1-AP (MORF4L2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human liver tissue using MORF4L2 Polyclonal antibody (14710-1-AP)

    WB analysis of human liver using 14710-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 14710-1-AP (MORF4L2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using MORF4L2 Polyclonal antibody (14710-1-AP)

    WB analysis of mouse liver using 14710-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 14710-1-AP (MORF4L2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using MORF4L2 Polyclonal antibody (14710-1-AP)

    IF Staining of MCF-7 using 14710-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using MORF4L2 antibody (14710-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using MORF4L2 Polyclonal antibody (14710-1-AP)

    IF Staining of HeLa using 14710-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 14710-1-AP (MORF4L2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

MORF4L2 Polyclonal antibody

MORF4L2 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 14710-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Mortality factor 4-like protein 2, mortality factor 4 like 2, MORF-related gene X protein, MORFL2, MORF related gene X protein

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, human liver tissue, mouse liver tissue, A431 cells, A549 cells, MCF-7 cells
Positive IF/ICC detected inMCF-7 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

14710-1-AP targets MORF4L2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag6404

Product name: Recombinant human MORF4L2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-288 aa of BC056899

Sequence: MSSRKQGSQPRGQQSAEEENFKKPTRSNMQRSKMRGASSGKKTAGPQQKNLEPALPGRWGGRSAENPPSGSVRKTRKNKQKTPGNGDGGSTSEAPQPPRKKRARADPTVESEEAFKNRMEVKVKIPEELKPWLVEDWDLVTRQKQLFQLPAKKNVDAILEEYANCKKSQGNVDNKEYAVNEVVAGIKEYFNVMLGTQLLYKFERPQYAEILLAHPDAPMSQVYGAPHLLRLFVRIGAMLAYTPLDEKSLALLLGYLHDFLKYLAKNSASLFTASDYKVASAEYHRKAL

相同性解析による交差性が予測される生物種
Full Name mortality factor 4 like 2
Calculated molecular weight 32 kDa
Observed molecular weight 30-32 kDa
GenBank accession numberBC056899
Gene Symbol MORF4L2
Gene ID (NCBI) 9643
RRIDAB_2297880
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ15014
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for MORF4L2 antibody 14710-1-APDownload protocol
WB protocol for MORF4L2 antibody 14710-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Front Immunol

Risk stratification based on DNA damage-repair-related signature reflects the microenvironmental feature, metabolic status and therapeutic response of breast cancer

Authors - Chunzhen Li
View Article
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