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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MORC3 Monoclonal antibody (68370-1-Ig)

    WB analysis using 68370-1-Ig (same clone as 68370-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68370-1-Ig (MORC3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 68370-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using MORC3 Monoclonal antibody (68370-1-Ig)

    WB analysis using 68370-1-Ig (same clone as 68370-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68370-1-Ig (MORC3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 68370-1-PBS in a different storage buffer formulation.

MORC3 Monoclonal antibody, PBS Only

MORC3 Monoclonal Antibody for WB, Indirect ELISA
Cat No. 68370-1-PBS
Clone No.1E1E1

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse

Applications

WB, Indirect ELISA

Synonyms

KIAA0136, MORC3, NXP2, ZCW5, ZCWCC3

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68370-1-PBS targets MORC3 in WB, Indirect ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag21867

Product name: Recombinant human MORC3 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 590-939 aa of BC132731

Sequence: DHDIDMKSEQSHVEQGGVQVEFVGDSEPCGQTGSTSTSSSRCDQGNTAATQTEVPSLVVKKEETVEDEIDVRNDAVILPSCVEAEAKIHETQETTDKSADDAGCQLQELRNQLLLVTEEKENYKRQCHMFTDQIKVLQQRILEMNDKYVKKETCHQSTETDAVFLLESINGKSESPDHMVSQYQQALEEIERLKKQCSALQHVKAECSQCSNNESKSEMDEMAVQLDDVFRQLDKCSIERDQYKSEVELLEMEKSQIRSQCEELKTEVEQLKSTNQQTATDVSTSSNIEESVNHMDGESLKLRSLRVNVGQLLAMIVPDLDLQQVNYDVDVVDEILGQVVEQMSEISST

相同性解析による交差性が予測される生物種
Full Name MORC family CW-type zinc finger 3
Calculated molecular weight 939 aa, 107 kDa
Observed molecular weight130 kDa
GenBank accession numberBC132731
Gene Symbol MORC3
Gene ID (NCBI) 23515
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDQ14149
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

MORC3, also named as MORC family CW-type zinc finger protein 3 or NXP2, is a 939 amino acid protein, which contains 1 CW-type zinc finger. MORC3 localizes in the nucleus and are expressed in heart, placenta, skeletal muscle, brain, pancreas, lung, liver, but not kidney. MORC3 as Nuclear factor which forms MORC3-NBs (nuclear bodies) via an ATP-dependent mechanism. Sumoylated MORC3-NBs can also associate with PML-NBs and recruits TP53 and SP100 to PML-NBs, thus regulating TP53 activity. It may be required for influenza A transcription during viral infection. The calculated molecular weight of MORC3 is a 107 kDa, but the Sumoylated MORC3 protein is about 130 kDa.