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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MON1B Monoclonal antibody (67985-1-Ig)

    WB analysis using 67985-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67985-1-Ig (MON1B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Western Blot (WB) analysis of A549 cells using MON1B Monoclonal antibody (67985-1-Ig)

    WB analysis of A549 using 67985-1-Ig

    WB result of MON1B antibody (67985-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MON1B transfected A549 cells.

  • Western Blot (WB) analysis of HeLa cells using MON1B Monoclonal antibody (67985-1-Ig)

    WB analysis of HeLa using 67985-1-Ig

    WB result of MON1B antibody (67985-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MON1B transfected HeLa cells.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using MON1B Monoclonal antibody (67985-1-Ig)

    IF Staining of HeLa using 67985-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using MON1B antibody (67985-1-Ig, Clone: 3E7C6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Featured Product
  • KD/KO Validated

MON1B Monoclonal antibody

MON1B Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 67985-1-Ig
Clone No.3E7C6

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Synonyms

HSRG1, KIAA0872, MON1 homolog B (yeast), MON1B, SAND2

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inLNCaP cells, HeLa cells, A549 cells, HEK-293 cells, Jurkat cells, HepG2 cells, K-562 cells, SW480 cells, MCF-7 cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67985-1-Ig targets MON1B in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag9920

Product name: Recombinant human MON1B protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 199-547 aa of BC024277

Sequence: STLTRASVARIFAHKQNYDLRRLLAGSERTLDRLLDSMEQDPGALLLGAVRCVPLARPLRDALGALLRRCTAPGLALSVLAVGGRLITAAQERNVLAECRLDPADLQLLLDWVGAPAFAAGEAWAPVCLPRFNPDGFFYAYVARLDAMPVCLLLLGTQREAFHAMAACRRLVEDGMHALGAMRALGEAASFSNASSASAPAYSVQAVGAPGLRHFLYKPLDIPDHHRQLPQFTSPELEAPYSREEERQRLSDLYHRLHARLHSTSRPLRLIYHVAEKETLLAWVTSKFELYTCLSPLVTKAGAILVVTKLLRWVKKEEDRLFIRYPPKYSTPPATSTDQAAHNGLFTGL

相同性解析による交差性が予測される生物種
Full Name MON1 homolog B (yeast)
Calculated molecular weight 547 aa, 59 kDa
Observed molecular weight59 kDa
GenBank accession numberBC024277
Gene Symbol MON1B
Gene ID (NCBI) 22879
RRIDAB_2918734
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ7L1V2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Vacuolar fusion protein MON1 homolog B (MON1B) is also named as HSRG1, KIAA0872 and SAND2, and belongs to the MON1/SAND family. The membrane recruitment of MON1B, a homolog of yeast MON1 protein in mammals, is essential for vesicular fusion of early endosomes (PMID:26987402).

Protocols

Product Specific Protocols
IF protocol for MON1B antibody 67985-1-IgDownload protocol
WB protocol for MON1B antibody 67985-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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