Validation Data Gallery
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Tested Applications
| Positive WB detected in | HEK-293 cells, K-562 cells, Jurkat cells, MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
23772-1-AP targets MON1A in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | MON1A fusion protein Ag6900 相同性解析による交差性が予測される生物種 |
| Full Name | MON1 homolog A (yeast) |
| Calculated molecular weight | 62 kDa |
| Observed molecular weight | 62 kDa and 45 kDa |
| GenBank accession number | BC047022 |
| Gene Symbol | MON1A |
| Gene ID (NCBI) | 84315 |
| RRID | AB_2879320 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q86VX9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
MON1A is a cytosolic protein that peripherally associates with the ER. MON1A plays an important role in membrane trafficking through the secretory apparatus. MON1A affects different steps in the secretory pathway including endoplasmic reticulum-to-Golgi traffic. MON1A encodes several isoforms with MW aboout 73, 62, 55, 45 and 34 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MON1A antibody 23772-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Death Dis TRIM39 deficiency inhibits tumor progression and autophagic flux in colorectal cancer via suppressing the activity of Rab7. | ||
Autophagy GORASP2 promotes phagophore closure and autophagosome maturation into autolysosomes |