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Species Reactivity

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Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using MOCS3 Polyclonal antibody (27501-1-AP)

    WB analysis of MCF-7 using 27501-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 27501-1-AP (MOCS3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using MOCS3 Polyclonal antibody (27501-1-AP)

    WB analysis of HepG2 using 27501-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 27501-1-AP (MOCS3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using MOCS3 Polyclonal antibody (27501-1-AP)

    IHC staining of human liver cancer using 27501-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27501-1-AP (MOCS3 antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using MOCS3 Polyclonal antibody (27501-1-AP)

    IHC staining of human liver cancer using 27501-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27501-1-AP (MOCS3 antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using MOCS3 Polyclonal antibody (27501-1-AP)

    IHC staining of human heart using 27501-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 27501-1-AP (MOCS3 antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using MOCS3 Polyclonal antibody (27501-1-AP)

    IHC staining of human heart using 27501-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 27501-1-AP (MOCS3 antibody) at dilution of 1:200 (under 40x lens).

MOCS3 Polyclonal antibody

MOCS3 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 27501-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IHC, ELISA

Synonyms

MOCS3, UBA4

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHepG2 cells, MCF-7 cells
Positive IHC detected inhuman liver cancer tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27501-1-AP targets MOCS3 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag26596

Product name: Recombinant human MOCS3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 279-404 aa of BC015939

Sequence: DALRGHFRSIRLRSRRLDCAACGERPTVTDLLDYEAFCGSSATDKCRSLQLLSPEERVSVTDYKRLLDSGAFHLLLDVRPQVEVDICRLPHALHIPLKHLERRDAESLKLLKEAIWEEKQGTQEGA

相同性解析による交差性が予測される生物種
Full Name molybdenum cofactor synthesis 3
Calculated molecular weight 50 kDa
Observed molecular weight 50 kDa, 65 kDa
GenBank accession numberBC015939
Gene Symbol MOCS3
Gene ID (NCBI) 27304
RRIDAB_2880892
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO95396
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MOCS3, also named as molybdenum cofactor synthesis 3 and UBA4. The human MOCS3 protein contains an N-terminal domain similar to the Escherichia coli MoeB protein and a C-terminal segment displaying similarities to the sulfurtransferase rhodanese. MOCS3 is proposed to catalyze both the adenylation and the subsequent generation of a thiocarboxylate group at the C-terminus of the smaller subunit of molybdopterin (MPT) synthase during Moco biosynthesis in humans (PMID: 15910006). MOCS3 has a calculated molecular weight of 50 kDa and can be detected another band of 65 kDa due to urmylation (PMID: 21209336).

Protocols

Product Specific Protocols
IHC protocol for MOCS3 antibody 27501-1-APDownload protocol
WB protocol for MOCS3 antibody 27501-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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