Validation Data Gallery
Tested Applications
| Positive WB detected in | A549 cells, LNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
68308-1-Ig targets MOCS2 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag6282 Product name: Recombinant human MOCS2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-188 aa of BC046097 Sequence: MSSLEISSSCFSLETKLPLSPPLVEDSAFEPSRKDMDEVEEKSKDVINFTAEKLSVDEVSQLVISPLCGAISLFVGTTRNNFEGKKVISLEYEAYLPMAENEVRKICSDIRQKWPVKHIAVFHRLGLVPVSEASIIIAVSSAHRAASLEAVSYAIDTLKAKVPIWKKEIYEESSTWKGNKECFWASNS 相同性解析による交差性が予測される生物種 |
| Full Name | molybdenum cofactor synthesis 2 |
| Calculated molecular weight | 21 kDa |
| Observed molecular weight | 21 kDa |
| GenBank accession number | BC046097 |
| Gene Symbol | MOCS2 |
| Gene ID (NCBI) | 4338 |
| RRID | AB_2935387 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | O96033 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
MOCS2(Molybdenum cofactor synthesis 2) is a heterotetrameric synthase comprised of 2 small (MOCS2A) and 2 large (MOCS2B) subunits. MOCS2 acts as a sulfur carrier required for molybdopterin biosynthesis. The two MOCS2 proteins A and B are subunits of molybdopterin synthase incorporating sulfur groups delivered by the MOCS3 sulfurylase.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for MOCS2 antibody 68308-1-Ig | Download protocol |
| WB protocol for MOCS2 antibody 68308-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
