Validation Data Gallery
Tested Applications
| Positive WB detected in | Jurkat cells, mouse liver tissue, rat liver tissue |
| Positive IF/ICC detected in | Jurkat cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
31550-1-AP targets MOCS1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag35879 Product name: Recombinant human MOCS1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 90-290 aa of NM_001075098 Sequence: EEGVPLTPKANLLTTEEILTLARLFVKEGIDKIRLTGGEPLIRPDVVDIVAQLQRLEGLRTIGVTTNGINLARLLPQLQKAGLSAINISLDTLVPAKFEFIVRRKGFHKVMEGIHKAIELGYNPVKVNCVVMRGLNEDELLDFAALTEGLPLDVRFIEYMPFDGNKWNFKKMVSYKEMLDTVRQQWPELEKVPEEESSTAK 相同性解析による交差性が予測される生物種 |
| Full Name | molybdenum cofactor synthesis 1 |
| Calculated molecular weight | 70kDa |
| Observed molecular weight | 40-43 kDa |
| GenBank accession number | NM_001075098 |
| Gene Symbol | MOCS1 |
| Gene ID (NCBI) | 4337 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q9NZB8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
MOCS1 (Molybdenum cofactor synthesis 1), also known as MOCS1A, MOCS1B, MOCOD, plays a crucial role in the biosynthesis of molybdenum cofactor and molybdopterin, which are essential for the activity of several key enzymes involved in carbon, nitrogen, and sulfur metabolism. MOCS1 produces two functional proteins, MOCS1A and MOCS1B, which follow different translocation routes before mitochondrial matrix import for cPMP biosynthesis involving both proteins (PMID: 31996372). MOCS1 was tumor suppressor gene affecting survival positively, while the other genes were oncogenes whose high expression was associated with poor survival (PMID: 37120564). The MOCS1 protein exists multiple isoforms.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for MOCS1 antibody 31550-1-AP | Download protocol |
| WB protocol for MOCS1 antibody 31550-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |