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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using MNK1 Polyclonal antibody (10136-1-AP)

    WB analysis of HeLa using 10136-1-AP (same clone as 10136-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 10136-1-AP (MNK1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10136-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HL-60 cells using MNK1 Polyclonal antibody (10136-1-AP)

    WB analysis of HL-60 using 10136-1-AP (same clone as 10136-1-PBS)

    HL-60 cells were subjected to SDS PAGE followed by western blot with 10136-1-AP (MNK1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10136-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using MNK1 Polyclonal antibody (10136-1-AP)

    WB analysis of HEK-293 using 10136-1-AP (same clone as 10136-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 10136-1-AP (MNK1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10136-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using MNK1 Polyclonal antibody (10136-1-AP)

    WB analysis of HepG2 using 10136-1-AP (same clone as 10136-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 10136-1-AP (MNK1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10136-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse small intestine tissue using MNK1 Polyclonal antibody (10136-1-AP)

    WB analysis of mouse small intestine using 10136-1-AP (same clone as 10136-1-PBS)

    mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 10136-1-AP (MNK1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10136-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using MNK1 Polyclonal antibody (10136-1-AP)

    IHC staining of mouse pancreas using 10136-1-AP (same clone as 10136-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 10136-1-AP (MNK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10136-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using MNK1 Polyclonal antibody (10136-1-AP)

    IHC staining of mouse pancreas using 10136-1-AP (same clone as 10136-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 10136-1-AP (MNK1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10136-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using MNK1 Polyclonal antibody (10136-1-AP)

    IF Staining of HepG2 using 10136-1-AP (same clone as 10136-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MNK1 antibody (10136-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 10136-1-PBS in a different storage buffer formulation.

MNK1 Polyclonal antibody, PBS Only

MNK1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 10136-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

MKNK1, EC:2.7.11.1, MAP kinase signal-integrating kinase 1, MAP kinase-interacting serine/threonine-protein kinase 1, MAPK signal-integrating kinase 1

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

10136-1-PBS targets MNK1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag0184

Product name: Recombinant human MKNK1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 299-465 aa of BC002755

Sequence: PPFVGHCGADCGWDRGEVCRVCQNKLFESIQEGKYEFPDKDWAHISSEAKDLISKLLVRDAKQRLSAAQVLQHPWVQGQAPEKGLPTPQVLQRNSSTMDLTLFAAEAIALNRQLSQHEENELAEEPEALADGLCSMKLSPPCKSRLARRRALAQAGRGEDRSPPTAL

相同性解析による交差性が予測される生物種
Full Name MAP kinase interacting serine/threonine kinase 1
Calculated molecular weight 51 kDa
Observed molecular weight 51 kDa, 47 kDa
GenBank accession numberBC002755
Gene Symbol MNK1
Gene ID (NCBI) 8569
RRIDAB_2250527
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BUB5
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

MNK1 (mitogen-activated protein kinase-interacting kinase 1) was discovered as a consequence of bacterial expression libraries screening for proteins regulated by the classical mitogen-activated protein kinases, the ERKs (extracellular signal-regulated kinases). Phosphorylation of Mnk1 has been shown to activate its kinase activity as well as to enhance its binding to the eukaryotic initiation factor 4G (eIF4G) which functions as a scaffolding protein. Human MNK1 arises from an alternatively spliced transcript giving rise to 2 isoforms, longer MNK1a (50 kDa) and a shorter MNK1b variant (38 kDa) that lacks 89 C-terminal amino acids(PMID: 21406405).

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