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Validation Data Gallery

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  • Western Blot (WB) analysis of K-562 cells using MNDA Polyclonal antibody (13576-1-AP)

    WB analysis of K-562 using 13576-1-AP (same clone as 13576-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 13576-1-AP (MNDA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13576-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HL-60 cells using MNDA Polyclonal antibody (13576-1-AP)

    WB analysis of HL-60 using 13576-1-AP (same clone as 13576-1-PBS)

    HL-60 cells were subjected to SDS PAGE followed by western blot with 13576-1-AP (MNDA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13576-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human bladder tissue using MNDA Polyclonal antibody (13576-1-AP)

    WB analysis of human bladder using 13576-1-AP (same clone as 13576-1-PBS)

    human bladder tissue were subjected to SDS PAGE followed by western blot with 13576-1-AP (MNDA antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13576-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach tissue using MNDA Polyclonal antibody (13576-1-AP)

    IHC staining of human stomach using 13576-1-AP (same clone as 13576-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 13576-1-AP (MNDA antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13576-1-PBS in a different storage buffer formulation.

MNDA Polyclonal antibody, PBS Only

MNDA Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 13576-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

PYHIN3

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

13576-1-PBS targets MNDA in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag4476

Product name: Recombinant human MNDA protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-407 aa of BC032319

Sequence: MVNEYKKILLLKGFELMDDYHFTSIKSLLAYDLGLTTKMQEEYNRIKITDLMEKKFQGVACLDKLIELAKDMPSLKNLVNNLRKEKSKVAKKIKTQEKAPVKKINQEEVGLAAPAPTARNKLTSEARGRIPVAQKRKTPNKEKTEAKRNKVSQEQSKPPGPSGASTSAAVDHPPLPQTSSSTPSNTSFTPNQETQAQRQVDARRNVPQNDPVTVVVLKATAPFKYESPENGKSTMFHATVASKTQYFHVKVFDINLKEKFVRKKVITISDYSECKGVMEIKEASSVSDFNQNFEVPNRIIEIANKTPKISQLYKQASGTMVYGLFMLQKKSVHKKNTIYEIQDNTGSMDVVGSGKWHNIKCEKGDKLRLFCLQLRTVDRKLKLVCGSHSFIKVIKAKKNKEGPMNVN

相同性解析による交差性が予測される生物種
Full Name myeloid cell nuclear differentiation antigen
Calculated molecular weight 407 aa, 46 kDa
Observed molecular weight 55 kDa
GenBank accession numberBC032319
Gene Symbol MNDA
Gene ID (NCBI) 4332
RRIDAB_2145071
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP41218
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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