Validation Data Gallery

Filter: AllWBIP

WB analysis of human liver using 11636-1-AP
human liver tissue were subjected to SDS PAGE followed by western blot with 11636-1-AP (MND1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis using 11636-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11636-1-AP (MND1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of human lung using 11636-1-AP
human lung tissue were subjected to SDS PAGE followed by western blot with 11636-1-AP (MND1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of human kidney using 11636-1-AP
human kidney tissue were subjected to SDS PAGE followed by western blot with 11636-1-AP (MND1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of human liver using 11636-1-AP
human liver tissue were subjected to SDS PAGE followed by western blot with 11636-1-AP (MND1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 11636-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 11636-1-AP (MND1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 11636-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 11636-1-AP (MND1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 11636-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 11636-1-AP (MND1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
IP experiment of mouse liver using 11636-1-AP
IP result of anti-MND1 (IP:11636-1-AP, 4ug; Detection:11636-1-AP 1:600) with mouse liver tissue lysate 6000ug.

Featured Product
KD/KO Validated

MND1 Polyclonal antibody

MND1 Polyclonal Antibody for WB, IP, ELISA

Cat No. 11636-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, IP, ELISA

Synonyms

GAJ, MND1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (3)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HepG2 cells, mouse liver tissue, human lung tissue, human kidney tissue, human liver tissue, MCF-7 cells
Positive IP detected in	mouse liver tissue

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:3000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 2 publications below
IF	See 1 publications below

Product Information

11636-1-AP targets MND1 in WB, IF, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag2210 Product name: Recombinant human MND1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-205 aa of BC032142 Sequence: MSKKKGLSAEEKRTRMMEIFSETKDVFQLKDLEKIAPKEKGITAMSVKEVLQSLVDDGMVDCERIGTSNYYWAFPSKALHARKHKLEVLESQLSEGSQKHASLQKSIEKAKIGRCETEERTRLAKELSSLRDQREQLKAEVEKYKDCDPQVVEEIRQANKVAKEAANRWTDNIFAIKSWAKRKFGFEENKIDRTFGIPEDFDYID 相同性解析による交差性が予測される生物種
Full Name	meiotic nuclear divisions 1 homolog (S. cerevisiae)
Calculated molecular weight	205 aa, 24 kDa
Observed molecular weight	30-35 kDa
GenBank accession number	BC032142
Gene Symbol	MND1
Gene ID (NCBI)	84057
RRID	AB_2266545
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9BWT6
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
IP protocol for MND1 antibody 11636-1-AP	Download protocol
WB protocol for MND1 antibody 11636-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IF	Oncotarget A study of meiomitosis and novel pathways of genomic instability in cutaneous T-cell lymphomas (CTCL). Authors - Matthew Tsang View Article
human	WB	Med Oncol Meiotic nuclear divisions 1 promotes proliferation and metastasis in hepatocellular carcinoma and is a potential diagnostic and therapeutic target gene Authors - Kai Tan KD Validated View Article
human	WB	J Inflamm Res Integrative Pan-Cancer Analysis Reveals the Oncogenic Role of MND1 and Validation of MND1's Role in Breast Cancer Authors - Wenwu Zhang KD Validated View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of human liver using 11636-1-AP

WB analysis using 11636-1-AP

WB analysis of human lung using 11636-1-AP

WB analysis of human kidney using 11636-1-AP

WB analysis of human liver using 11636-1-AP

WB analysis of HepG2 using 11636-1-AP

WB analysis of mouse liver using 11636-1-AP