Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, human brain tissue, PC-3 cells |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 17 publications below |
| CoIP | See 1 publications below |
Product Information
16015-1-AP targets MMS19 in WB, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag8792 Product name: Recombinant human MMS19 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 457-803 aa of BC006575 Sequence: FLDGNVSFLPENSFPSRFQPFQDGSSGQRRLIALLMAFVCSLPRNVEIPQLNQLMRELLELSCCHSCPFSSTAAAKCFAGLLNKHPAGQQLDEFLQLAVDKVEAGLDSGPCRSQAFTLLLWVTKALVLRYHPLSSCLTARLMGLLSDPELGPAAADGFSLLMSDCTDVLTRAGHAEVRIMFRQRFFTDNVPALVQGFHAAPQDVKPNYLKGLSHVLNRLPKPVLLPELPTLLSLLLEALSCPDCVVQLSTLSCLQPLLLEAPQVMSLHVDTLVTKFLNLSSSPSMAVRIAALQCMHALTRLPTPVLLPYKPQVIRALAKPLDDKKRLVRKEAVSARGEWFLLGSPGS 相同性解析による交差性が予測される生物種 |
| Full Name | MMS19 nucleotide excision repair homolog (S. cerevisiae) |
| Calculated molecular weight | 1030 aa, 113 kDa |
| Observed molecular weight | 103-113 kDa |
| GenBank accession number | BC006575 |
| Gene Symbol | MMS19 |
| Gene ID (NCBI) | 64210 |
| RRID | AB_2145043 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96T76 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
MMS19 plays a critical role in the biogenesis of multiple Fe-S proteins, which functions in genomic stability, RNA polymerase II function, and telomere length regulation. MMS19 has a alanine- and leucine-rich N terminus. It possible has a role in nucleotide excision repair(NER) and RNA polymerase II(POL II) transcription by interacting with ERCC2/XPB helicases, both subunits of NER-transcription factor TFIIH. Also MMS19 involves in the regulation of ER. It can function in chromosome segregation by acting as a part of the mitotic spindle-associated MMXD complex.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for MMS19 antibody 16015-1-AP | Download protocol |
| WB protocol for MMS19 antibody 16015-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Cell Cytosolic Iron-Sulfur Assembly Is Evolutionarily Tuned by a Cancer-Amplified Ubiquitin Ligase.
| ||
Cell Metab Human CIA2A-FAM96A and CIA2B-FAM96B Integrate Iron Homeostasis and Maturation of Different Subsets of Cytosolic-Nuclear Iron-Sulfur Proteins.
| ||
Nat Commun Structural basis for substrate recognition and chemical inhibition of oncogenic MAGE ubiquitin ligases. | ||
Cell Rep The CIA Targeting Complex Is Highly Regulated and Provides Two Distinct Binding Sites for Client Iron-Sulfur Proteins.
| ||
Mol Metab Cdkal1, a type 2 diabetes susceptibility gene, regulates mitochondrial function in adipose tissue. |
