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Validation Data Gallery

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  • Sandwich ELISA standard curve of MP01003-2

    Sandwich ELISA standard curve of MP01003-2

    Sandwich ELISA standard curve of MP01003-2, Human MMP-9 Recombinant Matched Antibody Pair - PBS only. 82685-18-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0442. 82685-16-PBS was HRP conjugated as the detection antibody. Range: 39-2500 pg/mL.

  • Sample test of MP01003-2

    Sample test of MP01003-2

    Serum of sixteen individual healthy human donors was measured. The MMP-9 concentration of detected samples was determined to be 213.0 ng/mL with a range of 67.5 - 426.4 ng/mL.

  • Sample test of MP01003-2

    Sample test of MP01003-2

    Saliva of eight individual healthy human donors was measured. The MMP-9 concentration of detected samples was determined to be 80.7 ng/mL with a range of 1.9 - 260.9 ng/mL.

  • Sample test of MP01003-2

    Sample test of MP01003-2

    Urine of eight individual healthy human donors was measured. The MMP-9 concentration of detected samples was determined to be 207.3 pg/mL with a range of 11.4 - 841.8 pg/mL.

  • Sample test of MP01003-2

    Sample test of MP01003-2

    Human peripheral blood mononuclear cells (PBMC) were cultured unstimulated or stimulated with 10 μg/mL PHA for 5 days. The mean MMP-9 concentration was determined to be 157.3 ng/mL in unstimulated PBMC supernatant, 237.9 ng/mL in PHA stimulated PBMC supernatant.

  • Cytometric bead array standard curve of MP01003-2

    Cytometric bead array standard curve of MP01003-2

    Cytometric bead array standard curve of MP01003-2, MMP-9 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 82685-18-PBS. Detection antibody: 82685-16-PBS. Standard: Eg0442. Range: 0.625-80 ng/mL.

  • Affinity and Kinetic Characterization of 82685-18-PBS

    Affinity and Kinetic Characterization of 82685-18-PBS

    Biolayer interferometry (BLl) kinetic assay of 82685-18-PBS against Human MMP-9 was performed. The affinity constant is below 1 pM.

MMP-9 Recombinant monoclonal antibody, PBS Only (Capture)

MMP-9 Uni-rAb® Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 82685-18-PBS
Clone No.241224C6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

MMP9, 67 kDa matrix metalloproteinase-9, 82 kDa matrix metalloproteinase-9, CLG4B, EC:3.4.24.35

Formulation:  PBS Only
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82685-18-PBS targets MMP-9 as part of a matched antibody pair:

MP01003-2: 82685-18-PBS capture and 82685-16-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Eg0442

Product name: Recombinant Human MMP9 protein (His Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: C-6*His

Domain: 20-707 aa of BC006093

Sequence: APRQRQSTLVLFPGDLRTNLTDRQLAEEYLYRYGYTRVAEMRGESKSLGPALLLLQKQLSLPETGELDSATLKAMRTPRCGVPDLGRFQTFEGDLKWHHHNITYWIQNYSEDLPRAVIDDAFARAFALWSAVTPLTFTRVYSRDADIVIQFGVAEHGDGYPFDGKDGLLAHAFPPGPGIQGDAHFDDDELWSLGKGVVVPTRFGNADGAACHFPFIFEGRSYSACTTDGRSDGLPWCSTTANYDTDDRFGFCPSERLYTRDGNADGKPCQFPFIFQGQSYSACTTDGRSDGYRWCATTANYDRDKLFGFCPTRADSTVMGGNSAGELCVFPFTFLGKEYSTCTSEGRGDGRLWCATTSNFDSDKKWGFCPDQGYSLFLVAAHEFGHALGLDHSSVPEALMYPMYRFTEGPPLHKDDVNGIRHLYGPRPEPEPRPPTTTTPQPTAPPTVCPTGPPTVHPSERPTAGPTGPPSAGPTGPPTAGPSTATTVPLSPVDDACNVNIFDAIAEIGNQLYLFKDGKYWRFSEGRGSRPQGPFLIADKWPALPRKLDSVFEERLSKKLFFFSGRQVWVYTGASVLGPRRLDKLGLGADVAQVTGALRSGRGKMLLFSGRRLWRFDVKAQMVDPRSASEVDRMFPGVPLDTHDVFQYREKAYFCQDRFYWRVSSRSELNQVDQVGYVTYDILQCPED

相同性解析による交差性が予測される生物種
Full Name matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase)
Calculated molecular weight 707 aa, 78 kDa
GenBank accession numberBC006093
Gene Symbol MMP-9
Gene ID (NCBI) 4318
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP14780
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.