Validation Data Gallery

Filter: AllWBIHCIF/ICC

WB analysis of A549 using 18237-1-AP (same clone as 18237-1-PBS)
A549 cells were subjected to SDS PAGE followed by western blot with 18237-1-AP (MMP28 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18237-1-PBS in a different storage buffer formulation.
WB analysis of mouse heart using 18237-1-AP (same clone as 18237-1-PBS)
mouse heart tissue were subjected to SDS PAGE followed by western blot with 18237-1-AP (MMP28 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18237-1-PBS in a different storage buffer formulation.
WB analysis of human colon using 18237-1-AP (same clone as 18237-1-PBS)
human colon tissue were subjected to SDS PAGE followed by western blot with 18237-1-AP (MMP28 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18237-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 18237-1-AP (same clone as 18237-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney using 18237-1-AP (MMP28 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 18237-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 18237-1-AP (same clone as 18237-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney using 18237-1-AP (MMP28 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 18237-1-PBS in a different storage buffer formulation.
IHC staining of human testis using 18237-1-AP (same clone as 18237-1-PBS)
Immunohistochemical analysis of paraffin-embedded human testis using 18237-1-AP (MMP28 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 18237-1-PBS in a different storage buffer formulation.
IHC staining of human testis using 18237-1-AP (same clone as 18237-1-PBS)
Immunohistochemical analysis of paraffin-embedded human testis using 18237-1-AP (MMP28 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 18237-1-PBS in a different storage buffer formulation.
IHC staining of human ovary using 18237-1-AP (same clone as 18237-1-PBS)
Immunohistochemical analysis of paraffin-embedded human ovary using 18237-1-AP (MMP28 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 18237-1-PBS in a different storage buffer formulation.
IHC staining of human ovary using 18237-1-AP (same clone as 18237-1-PBS)
Immunohistochemical analysis of paraffin-embedded human ovary using 18237-1-AP (MMP28 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 18237-1-PBS in a different storage buffer formulation.
IHC staining of human testis using 18237-1-AP (same clone as 18237-1-PBS)
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 18237-1-AP (MMP28 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 18237-1-PBS in a different storage buffer formulation.
IHC staining of human testis using 18237-1-AP (same clone as 18237-1-PBS)
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 18237-1-AP (MMP28 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 18237-1-PBS in a different storage buffer formulation.
IHC staining of human skin cancer using 18237-1-AP (same clone as 18237-1-PBS)
Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 18237-1-AP (MMP28 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 18237-1-PBS in a different storage buffer formulation.
IHC staining of human skin cancer using 18237-1-AP (same clone as 18237-1-PBS)
Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 18237-1-AP (MMP28 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 18237-1-PBS in a different storage buffer formulation.
IF Staining of A549 using 18237-1-AP (same clone as 18237-1-PBS)
Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using MMP28 antibody (18237-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 18237-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

MMP28 Polyclonal antibody, PBS Only

MMP28 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 18237-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

MM28, Matrix metalloproteinase-28, Matrix metalloproteinase 28, matrix metallopeptidase 28, Epilysin

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Overview

Tested Applications
Product Information

データシート［PDF］を印刷

MSDS

Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

18237-1-PBS targets MMP28 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag12980 Product name: Recombinant human MMP28 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 194-393 aa of BC002631 Sequence: LGNAFDGPGGALAHAFLPRRGEAHFDQDERWSLSRRRGRNLFVVLAHEIGHTLGLTHSPAPRALMAPYYKRLGRDALLSWDDVLAVQSLYGKPLGGSVAVQLPGKLFTDFETWDSYSPQGRRPETQGPKYCHSSFDAITVDRQQQLYIFKGSHFWEVAADGNVSEPRPLQERWVGLPPNIEAAAVSLNDGDFYFFKVQSV 相同性解析による交差性が予測される生物種
Full Name	matrix metallopeptidase 28
Calculated molecular weight	59 kDa
Observed molecular weight	48 kDa
GenBank accession number	BC002631
Gene Symbol	MMP28
Gene ID (NCBI)	79148
RRID	AB_2146444
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9H239
Storage Buffer	PBS only{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -80°C.

Background Information

MMP28(Epilysin)can degrade casein and play a role in tissues homeostasis and repair .The only specific human cell type recognized to express epilysin is the basal keratinocyte in the skin, where epilysin expression is upregulated during wound repair(PMID:16940349).Western blot showed this antibody recognize bands at 62 and 58 kDa as well as breakdown products at 50, 48, and 46 kDa(PMID:15953044).This antibody is specific to MMP28.

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of A549 using 18237-1-AP (same clone as 18237-1-PBS)

WB analysis of mouse heart using 18237-1-AP (same clone as 18237-1-PBS)

WB analysis of human colon using 18237-1-AP (same clone as 18237-1-PBS)

IHC staining of human kidney using 18237-1-AP (same clone as 18237-1-PBS)

IHC staining of human kidney using 18237-1-AP (same clone as 18237-1-PBS)

IHC staining of human testis using 18237-1-AP (same clone as 18237-1-PBS)

IHC staining of human testis using 18237-1-AP (same clone as 18237-1-PBS)

IHC staining of human ovary using 18237-1-AP (same clone as 18237-1-PBS)

IHC staining of human ovary using 18237-1-AP (same clone as 18237-1-PBS)

IHC staining of human testis using 18237-1-AP (same clone as 18237-1-PBS)

IHC staining of human testis using 18237-1-AP (same clone as 18237-1-PBS)

IHC staining of human skin cancer using 18237-1-AP (same clone as 18237-1-PBS)

IHC staining of human skin cancer using 18237-1-AP (same clone as 18237-1-PBS)

IF Staining of A549 using 18237-1-AP (same clone as 18237-1-PBS)

MMP28 Polyclonal antibody, PBS Only

MMP28 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

-/ -

Tested Applications

Recommended dilution

Product Information

Background Information

Able™

Able^™