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Species Reactivity

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Validation Data Gallery

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  • Western Blot (WB) analysis of SKOV-3 cells using MMP26 Polyclonal antibody (18087-1-AP)

    WB analysis of SKOV-3 using 18087-1-AP

    SKOV-3 cells were subjected to SDS PAGE followed by western blot with 18087-1-AP (MMP26 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human placenta tissue using MMP26 Polyclonal antibody (18087-1-AP)

    WB analysis of human placenta using 18087-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 18087-1-AP (MMP26 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using MMP26 Polyclonal antibody (18087-1-AP)

    WB analysis of HEK-293 using 18087-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 18087-1-AP (MMP26 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using MMP26 Polyclonal antibody (18087-1-AP)

    IHC staining of human prostate cancer using 18087-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer slide using 18087-1-AP (MMP26 Antibody) at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using MMP26 Polyclonal antibody (18087-1-AP)

    IHC staining of human prostate cancer using 18087-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer slide using 18087-1-AP (MMP26 Antibody) at dilution of 1:50.

  • Immunofluorescence (IF) / fluorescent staining of SKOV-3 cells using MMP26 Polyclonal antibody (18087-1-AP)

    IF Staining of SKOV-3 using 18087-1-AP

    Immunofluorescent analysis of SKOV-3 cells using 18087-1-AP (MMP26 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

MMP26 Polyclonal antibody

MMP26 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 18087-1-AP
Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Matrix metalloproteinase 26, matrix metallopeptidase 26, Matrilysin-2, Matrilysin 2, Endometase

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inSKOV-3 cells, HEK-293 cells, human placenta tissue
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSKOV-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18087-1-AP targets MMP26 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag12806

Product name: Recombinant human MMP26 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 24-262 aa of BC101541

Sequence: ADHKGWDFVEGYFHQFFLTKKESPLLTQETQTQLLQQFHRNGTDLLDMQMHALLHQPHCGVPDGSDTSISPGRCKWNKHTLTYRIINYPHDMKPSAVKDSIYNAVSIWSNVTPLIFQQVQNGDADIKVSFWQWAHEDGWPFDGPGGILGHAFLPNSGNPGVVHFDKNEHWSASDTGYNLFLVATHEIGHSLGLQHSGNQSSIMYPTYWYHDPRTFQLSADDIQRIQHLYGEKCSSDIP

相同性解析による交差性が予測される生物種
Full Name matrix metallopeptidase 26
Calculated molecular weight 262 aa, 30 kDa
Observed molecular weight 19 kDa
GenBank accession numberBC101541
Gene Symbol MMP26
Gene ID (NCBI) 56547
RRIDAB_2878495
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NRE1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MMP26(matrix metallopeptidase 26) may hydrolyze collagen type IV, fibronectin, fibrinogen, beta-casein, type I gelatin and alpha-1 proteinase inhibitor.MMP26 is expressed in placenta and uterus and is widely expressed in malignant tumors from different sources as well as in diverse tumor cell lines(PMID: 10987280).The proenzyme without the signal peptide has a calculated molecular mass of 29 kDa and the processed enzyme has a calculated molecular mass of 19 kDa(PMID:15574774).This antibody is specific to MMP26.

Protocols

Product Specific Protocols
IF protocol for MMP26 antibody 18087-1-APDownload protocol
IHC protocol for MMP26 antibody 18087-1-APDownload protocol
WB protocol for MMP26 antibody 18087-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Sci Adv

Succinate metabolism and membrane reorganization drives the endotheliopathy and coagulopathy of traumatic hemorrhage

Authors - Sarah Abdullah
View Article
humanIHC

J Huazhong Univ Sci Technolog Med Sci

MMP26: A potential biomarker for prostate cancer.

Authors - Teng Cheng
View Article
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