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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MMP25 Polyclonal antibody (30655-1-AP)

    WB analysis using 30655-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30655-1-AP (MMP25 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using MMP25 Polyclonal antibody (30655-1-AP)

    IHC staining of mouse spleen using 30655-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 30655-1-AP (MMP25 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using MMP25 Polyclonal antibody (30655-1-AP)

    IF Staining of A549 using 30655-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using MMP25 antibody (30655-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

MMP25 Polyclonal antibody

MMP25 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 30655-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

MTMMP6, MT6MMP, MT MMP6, MMP 25, matrix metallopeptidase 25

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHT-29 cells, Jurkat cells
Positive IHC detected inmouse spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30655-1-AP targets MMP25 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MMP25 fusion protein Ag29304 相同性解析による交差性が予測される生物種
Full Name matrix metallopeptidase 25
Calculated molecular weight 63 kDa
Observed molecular weight38-40 kDa
GenBank accession numberNM_022468
Gene Symbol MMP25
Gene ID (NCBI) 64386
RRIDAB_3669745
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NPA2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MMP25, also known as MT6-MMP, is one of the two glycosylphosphatidylinositol anchored matrix metalloproteinases (MMPs) that have been suggested to play a role in pericellular proteolysis. MMP25 was originally cloned from human leukocytes and its mRNA is predominantly expressed in leukocytes (polymorphonuclear cells and monocytes) of normal tissues and in brain, colon, urothelial and prostate cancers (PMID: 17513868). MMP-25 can exaggerate inflammatory responses by inactivating the main antiproteinase, a1-proteinase inhibitor or serpin (PMID: 16584348). The band at 57-kDa we detected is likely to be active MT6-MMP and minor MT6-MMP degradation product of 38-kDa.

Protocols

Product Specific Protocols
WB protocol for MMP25 antibody 30655-1-APDownload protocol
IHC protocol for MMP25 antibody 30655-1-APDownload protocol
IF protocol for MMP25 antibody 30655-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols