Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse liver tissue, L02 cells |
| Positive IF/ICC detected in | L02 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 2 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
Product Information
55289-1-AP targets MMP21 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | matrix metallopeptidase 21 |
| Calculated molecular weight | 65 kDa |
| Observed molecular weight | 67 kDa |
| GenBank accession number | NM_147191 |
| Gene Symbol | MMP21 |
| Gene ID (NCBI) | 118856 |
| RRID | AB_2881299 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8N119 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
MMP21 belongs to the peptidase M10A family. It may have an important and specific function in tumor progression and embryogenesis. It cleaves alpha-1-antitrypsin. The proenzyme without the signal peptide has a calculated molecular mass of 62 kD; following activation by furin, the processed enzyme has a calculated molecular mass of 49 kD(PMID:12617721),and MMP21 protein was detected in fetal neuroectoderm, in placenta, and in leukocytes(OMIM).This antibody is specific to MMP21.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MMP21 antibody 55289-1-AP | Download protocol |
| IF protocol for MMP21 antibody 55289-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun Wnt activation disturbs cell competition and causes diffuse invasion of transformed cells through NF-κB-MMP21 pathway | ||
Hum Genomics De novo disruptive heterozygous MMP21 variants are potential predisposing genetic risk factors in Chinese Han heterotaxy children | ||
Cancer Sci Matrix metalloproteinase-21 promotes metastasis via increasing the recruitment and M2 polarization of macrophages in HCC
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