Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis using 18165-1-AP (same clone as 18165-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 18165-1-AP (MMP13 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18165-1-PBS in a different storage buffer formulation.
WB analysis using 18165-1-AP (same clone as 18165-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 18165-1-AP (MMP13 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18165-1-PBS in a different storage buffer formulation.
WB analysis using 18165-1-AP (same clone as 18165-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 18165-1-AP (MMP13 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18165-1-PBS in a different storage buffer formulation.
IP experiment of MCF-7 using 18165-1-AP (same clone as 18165-1-PBS)
IP result of anti-MMP13 (IP:18165-1-AP, 4ug; Detection:18165-1-AP 1:300) with MCF-7 cells lysate 2480 ug. This data was developed using the same antibody clone with 18165-1-PBS in a different storage buffer formulation.
IHC staining of human liver using 18165-1-AP (same clone as 18165-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 18165-1-AP (MMP13 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18165-1-PBS in a different storage buffer formulation.
IHC staining of human liver using 18165-1-AP (same clone as 18165-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 18165-1-AP (MMP13 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18165-1-PBS in a different storage buffer formulation.
IHC staining of human hepatocirrhosis using 18165-1-AP (same clone as 18165-1-PBS)
Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 18165-1-AP (MMP13 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18165-1-PBS in a different storage buffer formulation.
IHC staining of human hepatocirrhosis using 18165-1-AP (same clone as 18165-1-PBS)
Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 18165-1-AP (MMP13 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18165-1-PBS in a different storage buffer formulation.
IHC staining of mouse liver using 18165-1-AP (same clone as 18165-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 18165-1-AP (MMP13 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18165-1-PBS in a different storage buffer formulation.
IHC staining of mouse liver using 18165-1-AP (same clone as 18165-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 18165-1-AP (MMP13 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18165-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 18165-1-AP (same clone as 18165-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18165-1-AP (MMP13 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18165-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 18165-1-AP (same clone as 18165-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18165-1-AP (MMP13 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18165-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 18165-1-AP (same clone as 18165-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer using 18165-1-AP (MMP13 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 18165-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 18165-1-AP (same clone as 18165-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer using 18165-1-AP (MMP13 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 18165-1-PBS in a different storage buffer formulation.
IF Staining of MCF-7 using 18165-1-AP (same clone as 18165-1-PBS)
Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using MMP13 antibody (18165-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 18165-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

MMP13 Polyclonal antibody, PBS Only

MMP13 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 18165-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

CLG3, Collagenase 3, EC:3.4.24.-, Matrix metalloproteinase 13, Matrix metalloproteinase-13

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information

データシート［PDF］を印刷

MSDS

Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

18165-1-PBS targets MMP13 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag12653 Product name: Recombinant human MMP13 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 100-471 aa of BC074808 Sequence: DVGEYNVFPRTLKWSKMNLTYRIVNYTPDMTHSEVEKAFKKAFKVWSDVTPLNFTRLHDGIADIMISFGIKEHGDFYPFDGPSGLLAHAFPPGPNYGGDAHFDDDETWTSSSKGYNLFLVAAHEFGHSLGLDHSKDPGALMFPIYTYTGKSHFMLPDDDVQGIQSLYGPGDEDPNPKHPKTPDKCDPSLSLDAITSLRGETMIFKDRFFWRLHPQQVDAELFLTKSFWPELPNRIDAAYEHPSHDLIFIFRGRKFWALNGYDILEGYPKKISELGLPKEVKKISAAVHFEDTGKTLLFSGNQVWRYDDTNHIMDKDYPRLIEEDFPGIGDKVDAVYEKNGYIYFFNGPIQFEYSIWSNRIVRVMPANSILWC 相同性解析による交差性が予測される生物種
Full Name	matrix metallopeptidase 13
Calculated molecular weight	471 aa, 54 kDa
Observed molecular weight	65-70 kDa, 50-55 kDa, 40 kDa
GenBank accession number	BC074808
Gene Symbol	MMP13
Gene ID (NCBI)	4322
RRID	AB_2144858
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P45452
Storage Buffer	PBS only{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -80°C.

Background Information

MMP-13(Matrix metalloproteinase-13), cleaves type I collagen and was previously thought to be the rodent analogue of human interstitial MMP-1. MMP-13 is found in hypertrophic and calcifying cartilage of mammalian growth plate. The bands seen with rat MMP-13 are consistent with a 59-kDa latent form and a 43-kDa active form reported for this enzyme, along with a smaller band at approximately 30 kDa(PMID:10771523). It also can be detected 3 bands that the glycosylated proMMP-13 (70 kDa), unglycosylated proform (55 kDa), and active MMP-13 enzyme (40kDa) by western blot(PMID:18332263).

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 18165-1-AP (same clone as 18165-1-PBS)

WB analysis using 18165-1-AP (same clone as 18165-1-PBS)

WB analysis using 18165-1-AP (same clone as 18165-1-PBS)

IP experiment of MCF-7 using 18165-1-AP (same clone as 18165-1-PBS)

IHC staining of human liver using 18165-1-AP (same clone as 18165-1-PBS)

IHC staining of human liver using 18165-1-AP (same clone as 18165-1-PBS)

IHC staining of human hepatocirrhosis using 18165-1-AP (same clone as 18165-1-PBS)

IHC staining of human hepatocirrhosis using 18165-1-AP (same clone as 18165-1-PBS)

IHC staining of mouse liver using 18165-1-AP (same clone as 18165-1-PBS)

IHC staining of mouse liver using 18165-1-AP (same clone as 18165-1-PBS)

IHC staining of human breast cancer using 18165-1-AP (same clone as 18165-1-PBS)

IHC staining of human breast cancer using 18165-1-AP (same clone as 18165-1-PBS)

IHC staining of human liver cancer using 18165-1-AP (same clone as 18165-1-PBS)

IHC staining of human liver cancer using 18165-1-AP (same clone as 18165-1-PBS)

IF Staining of MCF-7 using 18165-1-AP (same clone as 18165-1-PBS)

MMP13 Polyclonal antibody, PBS Only

MMP13 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

-/ -

Tested Applications

Recommended dilution

Product Information

Background Information

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