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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MMP11 Polyclonal antibody (30615-1-AP)

    WB analysis using 30615-1-AP (same clone as 30615-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30615-1-AP (MMP11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30615-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using MMP11 Polyclonal antibody (30615-1-AP)

    IF Staining of A549 using 30615-1-AP (same clone as 30615-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using MMP11 antibody (30615-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 30615-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using MMP11 Polyclonal antibody (30615-1-AP)

    IF Staining of MCF-7 using 30615-1-AP (same clone as 30615-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using MMP11 antibody (30615-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 30615-1-PBS in a different storage buffer formulation.

MMP11 Polyclonal antibody, PBS Only

MMP11 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 30615-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

30615-1-PBS targets MMP11 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag31191

Product name: Recombinant human MMP11 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 99-208 aa of NM_005940

Sequence: VLSGGRWEKTDLTYRILRFPWQLVQEQVRQTMAEALKVWSDVTPLTFTEVHEGRADIMIDFARYWHGDDLPFDGPGGILAHAFFPKTHREGDVHFDYDETWTIGDDQGTD

相同性解析による交差性が予測される生物種
Full Name matrix metallopeptidase 11 (stromelysin 3)
Calculated molecular weight 55 kDa
Observed molecular weight50-55 kDa
GenBank accession numberNM_005940
Gene Symbol MMP11
Gene ID (NCBI) 4320
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP24347
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Matrix metalloproteinase 11 (MMP11), also named stromelysin-3, is a member of the stromelysin subgroup belonging to MMPs superfamily. The role of MMP11 in cancer progression has been shown by several preclinical observations: its expression promotes tumor take in mice, homing of malignant epithelial cells, cancer progression by remodeling extracellular matrix, and antiapoptotic and antinecrotic effect on tumor cells (PMID: 19509157, 26892540). MMP11 has a calculated molecular mass of 55 kDa.

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