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Validation Data Gallery

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  • Sandwich ELISA standard curve of MP00290-1

    Sandwich ELISA standard curve of MP00290-1

    Sandwich ELISA standard curve of MP00290-1, MMP1 Recombinant Matched Antibody Pair, PBS Only. 83114-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard  Eg0527. 83114-3-PBS was HRP conjugated as the detection antibody. Range: 19.5-1250 pg/mL.

  • Sample test of MP00290-1

    Sample test of MP00290-1

    Plasma of fourteen individual healthy human donors were measured. The human MMP1 concentration of detected samples was determined to be 653.4 pg/mL with a range of 99.3 - 1,631.8 pg/mL.

  • Sample test of MP00290-1

    Sample test of MP00290-1

    HT1080 were cultured in DMEM supplemented with 10% fetal bovine serum, 2.5 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernate was removed, assayed for human MMP1, and measured 907.5 pg/mL.

  • Sample test of MP00290-1

    Sample test of MP00290-1

    Serum of fourteen individual healthy human donors were measured. The human MMP1 concentration of detected samples was determined to be 9,124.3 pg/mL with a range of 3,045.5 - 19,864.3 pg/mL.

  • Cytometric bead array standard curve of MP00290-1

    Cytometric bead array standard curve of MP00290-1

    Cytometric bead array standard curve of MP00290-1, MMP1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83114-4-PBS. Detection antibody: 83114-3-PBS. Standard: Eg0527. Range: 0.625-20 ng/mL.

  • Affinity and Kinetic Characterization of 83114-3-PBS

    Affinity and Kinetic Characterization of 83114-3-PBS

    Biolayer interferometry (BLl) kinetic assay of 83114-3-PBS against Human MMP1 was performed. The affinity constant is 12.7 nM.

MMP1 Recombinant monoclonal antibody, PBS Only (Detector)

MMP1 Uni-rAb® Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 83114-3-PBS
Clone No.230343B6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

22 kDa interstitial collagenase, 27 kDa interstitial collagenase, CLG, CLGN, EC:3.4.24.7

Formulation:  PBS Only
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83114-3-PBS targets MMP1 as part of a matched antibody pair:

MP00290-1: 83114-4-PBS capture and 83114-3-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Eg0527

Product name: Recombinant human MMP1 protein

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: C-6*HIS

Domain: 20-469 aa of BC013875

Sequence: FPATLETQEQDVDLVQKYLEKYYNLKNDGRQVEKRRNSGPVVEKLKQMQEFFGLKVTGKPDAETLKVMKQPRCGVPDVAQFVLTEGNPRWEQTHLTYRIENYTPDLPRADVDHAIEKAFQLWSNVTPLTFTKVSEGQADIMISFVRGDHRDNSPFDGPGGNLAHAFQPGPGIGGDAHFDEDERWTNNFREYNLHRVAAHELGHSLGLSHSTDIGALMYPSYTFSGDVQLAQDDIDGIQAIYGRSQNPVQPIGPQTPKACDSKLTFDAITTIRGEVMFFKDRFYMRTNPFYPEVELNFISVFWPQLPNGLEAAYEFADRDEVRFFKGNKYWAVQGQNVLHGYPKDIYSSFGFPRTVKHIDAALSEENTGKTYFFVANKYWRYDEYKRSMDPGYPKMIAHDFPGIGHKVDAVFMKDGFFYFFHGTRQYKFDPKTKRILTLQKANSWFNCRKN

相同性解析による交差性が予測される生物種
Full Name matrix metallopeptidase 1 (interstitial collagenase)
Calculated molecular weight 54 kDa
GenBank accession numberBC013875
Gene Symbol MMP1
Gene ID (NCBI) 4312
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP03956
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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