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Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using MMP-9 (N-terminal) Polyclonal antibody (10375-2-AP)

    WB analysis using 10375-2-AP (same clone as 10375-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10375-2-AP (MMP9 (N-terminal) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10375-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using MMP-9 (N-terminal) Polyclonal antibody (10375-2-AP)

    WB analysis using 10375-2-AP (same clone as 10375-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10375-2-AP (MMP-9 (N-terminal) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10375-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using MMP-9 (N-terminal) Polyclonal antibody (10375-2-AP)

    WB analysis using 10375-2-AP (same clone as 10375-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10375-2-AP (MMP9 (N-terminal) antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10375-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of U2OS cells using MMP-9 (N-terminal) Polyclonal antibody (10375-2-AP)

    WB analysis of U2OS using 10375-2-AP (same clone as 10375-2-PBS)

    WB result of MMP-9 (N-terminal) antibody (10375-2-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MMP-9 (N-terminal) transfected U2OS cells. This data was developed using the same antibody clone with 10375-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using MMP-9 (N-terminal) Polyclonal antibody (10375-2-AP)

    WB analysis using 10375-2-AP (same clone as 10375-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10375-2-AP (MMP9 (N-terminal) antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10375-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using MMP-9 (N-terminal) Polyclonal antibody (10375-2-AP)

    IHC staining of human cervical cancer using 10375-2-AP (same clone as 10375-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10375-2-AP (MMP9 (N-terminal) antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10375-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using MMP-9 (N-terminal) Polyclonal antibody (10375-2-AP)

    IHC staining of human cervical cancer using 10375-2-AP (same clone as 10375-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10375-2-AP (MMP9 (N-terminal) antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10375-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using MMP-9 (N-terminal) Polyclonal antibody (10375-2-AP)

    IHC staining of human breast cancer using 10375-2-AP (same clone as 10375-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10375-2-AP (MMP9 (N-terminal) antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 10375-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using MMP-9 (N-terminal) Polyclonal antibody (10375-2-AP)

    IHC staining of human breast cancer using 10375-2-AP (same clone as 10375-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10375-2-AP (MMP9 (N-terminal) antibody at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 10375-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using MMP-9 (N-terminal) Polyclonal antibody (10375-2-AP)

    IHC staining of human breast cancer using 10375-2-AP (same clone as 10375-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10375-2-AP (MMP9 (N-terminal) antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10375-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using MMP-9 (N-terminal) Polyclonal antibody (10375-2-AP)

    IHC staining of human breast cancer using 10375-2-AP (same clone as 10375-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10375-2-AP (MMP9 (N-terminal) antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10375-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using MMP-9 (N-terminal) Polyclonal antibody (10375-2-AP)

    IHC staining of human breast cancer using 10375-2-AP (same clone as 10375-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10375-2-AP (MMP9 (N-terminal) antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10375-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using MMP-9 (N-terminal) Polyclonal antibody (10375-2-AP)

    IHC staining of human breast cancer using 10375-2-AP (same clone as 10375-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10375-2-AP (MMP9 (N-terminal) antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10375-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using MMP-9 (N-terminal) Polyclonal antibody (10375-2-AP)

    IHC staining of human cervical cancer using 10375-2-AP (same clone as 10375-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10375-2-AP (MMP9 (N-terminal) antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10375-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using MMP-9 (N-terminal) Polyclonal antibody (10375-2-AP)

    IHC staining of human cervical cancer using 10375-2-AP (same clone as 10375-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10375-2-AP (MMP9 (N-terminal) antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10375-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using MMP-9 (N-terminal) Polyclonal antibody (10375-2-AP)

    IF Staining of HepG2 using 10375-2-AP (same clone as 10375-2-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MMP9 (N-terminal) antibody (10375-2-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 10375-2-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using MMP-9 (N-terminal) Polyclonal antibody (10375-2-AP)

    FC experiment of HeLa using 10375-2-AP (same clone as 10375-2-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human MMP9 (N-terminal) (10375-2-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 10375-2-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

MMP-9 (N-terminal) Polyclonal antibody, PBS Only

MMP-9 (N-terminal) Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 10375-2-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Synonyms

MMP9 (N-terminal), MMP9, 67 kDa matrix metalloproteinase-9, 82 kDa matrix metalloproteinase-9, CLG4B

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

10375-2-PBS targets MMP-9 (N-terminal) in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag0552

Product name: Recombinant human MMP9 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-100 aa of BC006093

Sequence: MSLWQPLVLVLLVLGCCFAAPRQRQSTLVLFPGDLRTNLTDRQLAEEYLYRYGYTRVAEMRGESKSLGPALLLLQKQLSLPETGELDSATLKAMRTPRCG

相同性解析による交差性が予測される生物種
Full Name matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase)
Calculated molecular weight 707 aa, 78 kDa
Observed molecular weight92 kDa, 67 kDa
GenBank accession numberBC006093
Gene Symbol MMP-9
Gene ID (NCBI) 4318
RRIDAB_10897178
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP14780
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, tissue remodeling, and disease processes, such as arthritis or metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. Matrix metalloproteinase 9 (gelatinase B, 92 kDa gelatinase, 92 kDa type IV collagenase) (MMP9, synonyms: GELB, CLG4B) degrades collagens type IV and V. Studies in rhesus monkeys suggest that MMP9 is involved in IL-8-induced mobilization hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. The pro-MMP9 is 92 kDa, and it can be detected a processed form of 68 kDa. This protein can exist as a dimer of 180 kDa (PMID:7492685).

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