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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MMGT1 Polyclonal antibody (31166-1-AP)

    WB analysis using 31166-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31166-1-AP (MMGT1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using MMGT1 Polyclonal antibody (31166-1-AP)

    IHC staining of mouse kidney using 31166-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 31166-1-AP (MMGT1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using MMGT1 Polyclonal antibody (31166-1-AP)

    IHC staining of mouse kidney using 31166-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 31166-1-AP (MMGT1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using MMGT1 Polyclonal antibody (31166-1-AP)

    IHC staining of human colon cancer using 31166-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 31166-1-AP (MMGT1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using MMGT1 Polyclonal antibody (31166-1-AP)

    IHC staining of human colon cancer using 31166-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 31166-1-AP (MMGT1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse adrenal gland tissue using MMGT1 Polyclonal antibody (31166-1-AP)

    IHC staining of mouse adrenal gland using 31166-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse adrenal gland tissue slide using 31166-1-AP (MMGT1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse colon tissue using MMGT1 Polyclonal antibody (31166-1-AP)

    IHC staining of mouse colon using 31166-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 31166-1-AP (MMGT1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat adrenal gland tissue using MMGT1 Polyclonal antibody (31166-1-AP)

    IHC staining of rat adrenal gland using 31166-1-AP

    Immunohistochemical analysis of paraffin-embedded rat adrenal gland tissue slide using 31166-1-AP (MMGT1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat adrenal gland tissue using MMGT1 Polyclonal antibody (31166-1-AP)

    IHC staining of rat adrenal gland using 31166-1-AP

    Immunohistochemical analysis of paraffin-embedded rat adrenal gland tissue slide using 31166-1-AP (MMGT1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat colon tissue using MMGT1 Polyclonal antibody (31166-1-AP)

    IHC staining of rat colon using 31166-1-AP

    Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 31166-1-AP (MMGT1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat kidney tissue using MMGT1 Polyclonal antibody (31166-1-AP)

    IHC staining of rat kidney using 31166-1-AP

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 31166-1-AP (MMGT1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

MMGT1 Polyclonal antibody

MMGT1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 31166-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

TMEM32, Transmembrane protein 32

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inMDA-MB-468 cells, PC-3 cells, SW480 cells, U-251 cells
Positive IHC detected inrat adrenal gland tissue, mouse kidney tissue, human colon cancer tissue, mouse adrenal gland tissue, mouse colon tissue, rat colon tissue, rat kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31166-1-AP targets MMGT1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag35121

Product name: Recombinant human MMGT1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 65-131 aa of BC033588

Sequence: GEFKDMDATSELKNKTFDTLRNHPSFYVFNHRGRVLFRPSDTANSSNQDALSSNTSLKLRKLESLRR

相同性解析による交差性が予測される生物種
Full Name membrane magnesium transporter 1
Calculated molecular weight 131 aa, 15 kDa
Observed molecular weight15-20 kDa
GenBank accession numberBC033588
Gene Symbol MMGT1
Gene ID (NCBI) 93380
RRIDAB_3742401
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ8N4V1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MMGT1 (Membrane Magnesium Transporter 1) is a protein encoded by the MMGT1 gene in humans. It is also known by the aliases EMC5 and TMEM32. This protein is a member of the magnesium transporter family and is involved in the transport of magnesium ions across cell membranes.

Protocols

Product Specific Protocols
IHC protocol for MMGT1 antibody 31166-1-APDownload protocol
WB protocol for MMGT1 antibody 31166-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

EMBO J

Principles of ribosome-associated protein quality control during the synthesis of CFTR.

Authors - Tom Joshua Oldfield
View Article
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