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Validation Data Gallery

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  • Western Blot (WB) analysis of Raji cells using MMACHC Polyclonal antibody (21462-1-AP)

    WB analysis of Raji using 21462-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 21462-1-AP (MMACHC Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using MMACHC Polyclonal antibody (21462-1-AP)

    WB analysis of HeLa using 21462-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 21462-1-AP (MMACHC antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using MMACHC Polyclonal antibody (21462-1-AP)

    IHC staining of human liver using 21462-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 21462-1-AP (MMACHC antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using MMACHC Polyclonal antibody (21462-1-AP)

    IHC staining of human liver using 21462-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 21462-1-AP (MMACHC antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

MMACHC Polyclonal antibody

MMACHC Polyclonal Antibody for WB, IHC, ELISA
Cat No. 21462-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

cblC, MMACHC, RP11 291L19.3

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inRaji cells, HeLa cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21462-1-AP targets MMACHC in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag15001

Product name: Recombinant human MMACHC protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-225 aa of BC006122

Sequence: MFDRALKPFLQSCHLRMLTDPVDQCVAYHLGRVRESLPELQIEIIADYEVHPNRRPKILAQTAAHVAGAAYYYQRQDVEADPWGNQRISGVCIHPRFGGWFAIRGVVLLPGIEVPDLPPRKPHDCVPTRADRIALLEGFNFHWRDWTYRDAVTPQERYSEEQKAYFSTPPAQRLALLGLAQPSEKPSSPSPDLPFTTPAPKKPGNPSRARSWLSPRVSPPASPGP

相同性解析による交差性が予測される生物種
Full Name methylmalonic aciduria (cobalamin deficiency) cblC type, with homocystinuria
Calculated molecular weight 282 aa, 32 kDa
Observed molecular weight 32 kDa
GenBank accession numberBC006122
Gene Symbol MMACHC
Gene ID (NCBI) 25974
RRIDAB_10732959
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y4U1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MMACHC, also named as CblC, is located on chromosome 1 and comprises four coding exons. Human MMACHC catalyzes the reductive decyanation of cyanocobalamin and may serve as a trafficking chaperone for intracellular cobalamin. It also catalyzes the glutathione-dependent reductive demethylation of methylcobalamin, and, with much lower efficiency, the glutathione-dependent reductive demethylation of adenosylcobalamin. NMACHC binds cyanocobalamin, adenosylcobalamin, methylcobalamin and other, related vitamin B12 derivatives.

Protocols

Product Specific Protocols
WB protocol for MMACHC antibody 21462-1-APDownload protocol
IHC protocol for MMACHC antibody 21462-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols