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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MLLT1 Polyclonal antibody (31029-1-AP)

    WB analysis using 31029-1-AP (same clone as 31029-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 31029-1-AP (MLLT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31029-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using MLLT1 Polyclonal antibody (31029-1-AP)

    WB analysis of HeLa using 31029-1-AP (same clone as 31029-1-PBS)

    WB result of MLLT1 antibody (31029-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MLLT1 transfected HeLa cells. This data was developed using the same antibody clone with 31029-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using MLLT1 Polyclonal antibody (31029-1-AP)

    IF Staining of HeLa using 31029-1-AP (same clone as 31029-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MLLT1 antibody (31029-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 31029-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

31029-1-PBS targets MLLT1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MLLT1 fusion protein Ag34210 相同性解析による交差性が予測される生物種
Full Name myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 1
Calculated molecular weight62 kDa
Observed molecular weight80 kDa
GenBank accession numberBC148806
Gene Symbol MLLT1
Gene ID (NCBI) 4298
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ03111
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.