Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, HepG2 cells, PC-3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
87741-1-RR targets MLKL in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag15188 Product name: Recombinant human MLKL protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-263 aa of BC028141 Sequence: MENLKHIITLGQVIHKRCEEMKYCKKQCRRLGHRVLGLIKPLEMLQDQGKRSVPSEKLTTAMNRFKAALEEANGEIEKFSNRSNICRFLTASQDKILFKDVNRKLSDVWKELSLLLQVEQRMPVSPISQGASWAQEDQQDADEDRRAFQMLRRDNEKIEASLRRLEINMKEIKETLRQSLESSSGKSPLEISRFKVKNVKTGSASGCNSEKIRKLVAVKRQQEPLGEDCPSELREIIDECRAHDPSVRPSVDEILKKLSTFSK 相同性解析による交差性が予測される生物種 |
| Full Name | mixed lineage kinase domain-like |
| Calculated molecular weight | 471 aa, 54 kDa |
| Observed molecular weight | 55 kDa |
| GenBank accession number | BC028141 |
| Gene Symbol | MLKL |
| Gene ID (NCBI) | 197259 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q8NB16 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
MLKL is a pseudokinase effector of necroptosis that becomes activated through RIPK3-mediated phosphorylation, leading to oligomerization and plasma membrane disruption. By executing necroptotic cell death and promoting release of inflammatory mediators, MLKL plays a central role in innate immunity, inflammatory pathology, and host defense. Dysregulation of MLKL-dependent necroptosis has been linked to inflammatory disorders, infection, and cancer-related immune responses.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MLKL antibody 87741-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |