Validation Data Gallery
Tested Applications
| Positive IHC-Autostainer detected in | human ovary cancer tissue, human stomach cancer tissue, human tonsillitis tissue, human appendicitis tissue |
| Positive IHC detected in | Human tonsillitis tissue, human stomach cancer tissue, human ovary cancer tissue, human colorectal cancer tissue, human rectal cancer tissue, human appendicitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC)-AUTOSTAINER | IHC-AUTOSTAINER : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
60822-2-Ig targets MLH1 in IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag27723 Product name: Recombinant human MLH1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 407-756 aa of BC006850 Sequence: QPQAIVTEDKTDISSGRARQQDEEMLELPAPAEVAAKNQSLEGDTTKGTSEMSEKRGPTSSNPRKRHREDSDVEMVEDDSRKEMTAACTPRRRIINLTSVLSLQEEINEQGHEVLREMLHNHSFVGCVNPQWALAQHQTKLYLLNTTKLSEELFYQILIYDFANFGVLRLSEPAPLFDLAMLALDSPESGWTEEDGPKEGLAEYIVEFLKKKAEMLADYFSLEIDEEGNLIGLPLLIDNYVPPLEGLPIFILRLATEVNWDEEKECFESLSKECAMFYSIRKQYISEESTLSGQQSEVPGSIPNSWKWTVEHIVYKALRSHILPPKHFTEDGNILQLANLPDLYKVFERC 相同性解析による交差性が予測される生物種 |
| Full Name | mutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli) |
| Calculated molecular weight | 756 aa, 85 kDa |
| GenBank accession number | BC006850 |
| Gene Symbol | MLH1 |
| Gene ID (NCBI) | 4292 |
| RRID | AB_3743234 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P40692 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
MLH1, also named as COCA2, belongs to the DNA mismatch repair mutL/hexB family. It heterodimerizes with PMS2 to form MutL alpha which is a component of the post-replicative DNA mismatch repair system (MMR). MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. MLH1 also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. MLH1 heterodimerizes with MLH3 to form MutL gamma which plays a role in meiosis.(PMID: 16873062, PMID: 18206974) Defects in MLH1 are the cause of hereditary non-polyposis colorectal cancer type 2 (HNPCC2). Defects in MLH1 are a cause of mismatch repair cancer syndrome (MMRCS). Defects in MLH1 are a cause of Muir-Torre syndrome (MTS). Defects in MLH1 are a cause of susceptibility to endometrial cancer.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for MLH1 antibody 60822-2-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |