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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MLF1IP Polyclonal antibody (13186-1-AP)

    WB analysis using 13186-1-AP (same clone as 13186-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 13186-1-AP (MLF1IP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13186-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using MLF1IP Polyclonal antibody (13186-1-AP)

    WB analysis using 13186-1-AP (same clone as 13186-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 13186-1-AP (MLF1IP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13186-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using MLF1IP Polyclonal antibody (13186-1-AP)

    WB analysis using 13186-1-AP (same clone as 13186-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 13186-1-AP (MLF1IP antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13186-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using MLF1IP Polyclonal antibody (13186-1-AP)

    IHC staining of mouse colon using 13186-1-AP (same clone as 13186-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 13186-1-AP (MLF1IP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13186-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using MLF1IP Polyclonal antibody (13186-1-AP)

    IHC staining of mouse colon using 13186-1-AP (same clone as 13186-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 13186-1-AP (MLF1IP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13186-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using MLF1IP Polyclonal antibody (13186-1-AP)

    IHC staining of human colon using 13186-1-AP (same clone as 13186-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 13186-1-AP (MLF1IP Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 13186-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using MLF1IP Polyclonal antibody (13186-1-AP)

    IHC staining of human colon using 13186-1-AP (same clone as 13186-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 13186-1-AP (MLF1IP Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 13186-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using MLF1IP Polyclonal antibody (13186-1-AP)

    IHC staining of human gliomas using 13186-1-AP (same clone as 13186-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13186-1-AP (MLF1IP Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 13186-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using MLF1IP Polyclonal antibody (13186-1-AP)

    IHC staining of human gliomas using 13186-1-AP (same clone as 13186-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13186-1-AP (MLF1IP Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 13186-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using MLF1IP Polyclonal antibody (13186-1-AP)

    IF Staining of HepG2 using 13186-1-AP (same clone as 13186-1-PBS)

    Immunofluorescent analysis of HepG2 cells using 13186-1-AP (MLF1IP antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13186-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

MLF1IP Polyclonal antibody, PBS Only

MLF1IP Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 13186-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

CENP 50, CENP U, CENP U(50), CENP50, CENP-50

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

13186-1-PBS targets MLF1IP in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag3858

Product name: Recombinant human MLF1IP protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 243-418 aa of BC031520

Sequence: MKTSDIKELNIVLPEFEKTHLEHQQRIESKVCKAAIATFYVNVKEQFIKMLKESQMLTNLKRKNAKMISDIEKKRQRMIEVQDELLRLEPQLKQLQTKYDELKERKSSLRNAAYFLSNLKQLYQDYSDVQAQEPNVKETYDSSSLPALLFKARTLLGAESHLRNINHQLEKLLDQG

相同性解析による交差性が予測される生物種
Full Name MLF1 interacting protein
Calculated molecular weight 418 aa, 48 kDa
Observed molecular weight 46-50 kDa, 60 kDa
GenBank accession numberBC031520
Gene Symbol MLF1IP
Gene ID (NCBI) 79682
RRIDAB_10642941
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ71F23
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

MLF1IP, also named as CENPU, ICEN24, KLIP1 and PBIP1, belongs to the CENP-U/AME1 family. MLF1IP plays an important role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. Moreover, MLF1IP has been shown to be involved in tumorigenesis in several types of cancer including glioblastoma, prostate cancer and luminal breast cancer (PMID: 27378428). MLF1IP has 3 isoforms with the molecular mass of 21, 38 and 47 kDa.

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