Validation Data Gallery
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Tested Applications
| Positive WB detected in | HepG2 cells, MCF-7 cells, MDA-MB-231 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
27012-1-AP targets MKX in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | MKX fusion protein Ag25549 相同性解析による交差性が予測される生物種 |
| Full Name | mohawk homeobox |
| Calculated molecular weight | 352 aa, 39 kDa |
| Observed molecular weight | 40 kDa |
| GenBank accession number | BC036207 |
| Gene Symbol | MKX |
| Gene ID (NCBI) | 283078 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8IYA7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Mohawk (Mkx) is a member of the Three Amino Acid Loop Extension (TALE) superclass of atypical homeobox genes. It encodes a transcriptional repressor that is expressed in the embryonic precursors of skeletal muscle, tendon, cartilage, and bone. This gene is highly conserved across species and is known to be involved in the differentiation and maintenance of these tissues.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MKX antibody 27012-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |