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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MKRN3 Polyclonal antibody

MKRN3 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 18349-1-AP

Synonyms

D15S9, makorin ring finger protein 3, MKRN3, RING finger protein 63, RNF63, ZFP127, Zinc finger protein 127, ZNF127

Validation Data Gallery

Applications:
  • WB analysis using 18349-1-AP

    WB analysis using 18349-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18349-1-AP (MKRN3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse colon using 18349-1-AP

    IHC staining of mouse colon using 18349-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 18349-1-AP (MKRN3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse colon using 18349-1-AP

    IHC staining of mouse colon using 18349-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 18349-1-AP (MKRN3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of A431 using 18349-1-AP

    IF Staining of A431 using 18349-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using MKRN3 antibody (18349-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inmouse liver tissue
Positive IHC detected inmouse colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

18349-1-AP targets MKRN3 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MKRN3 fusion protein Ag13167 相同性解析による交差性が予測される生物種
Full Name makorin ring finger protein 3
Calculated molecular weight 507 aa, 56 kDa
Observed molecular weight 56 kDa
GenBank accession numberBC044639
Gene symbol MKRN3
Gene ID (NCBI) 7681
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for MKRN3 antibody 18349-1-APDownload protocol
IHC protocol for MKRN3 antibody 18349-1-APDownload protocol
IF protocol for MKRN3 antibody 18349-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols