Validation Data Gallery
Tested Applications
Recommended dilution
| Application | Dilution |
|---|---|
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Product Information
29853-1-PBS targets MIST1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag32096 Product name: Recombinant human BHLHA15 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-189 aa of NM_177455 Sequence: MKTKNRPPRRRAPVQDTEATPGEGTPDGSLPNPGPEPAKGLRSRPARAAARAPGEGRRRRPGPSGPGGRRDSSIQRRLESNERERQRMHKLNNAFQALREVIPHVRADKKLSKIETLTLAKNYIKSLTATILTMSSSRLPGLEGPGPKLYQHYQQQQQVAGGALGATEAQPQGHLQRYSTQIHSFREGT* 相同性解析による交差性が予測される生物種 |
| Full Name | basic helix-loop-helix family, member a15 |
| Calculated molecular weight | 21 kDa |
| Observed molecular weight | 20-30 kDa |
| GenBank accession number | NM_177455 |
| Gene Symbol | MIST1 |
| Gene ID (NCBI) | 168620 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q7RTS1 |
| Storage Buffer | PBS only{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -80°C. |
Background Information
Muscle intestine stomach expression 1 (MIST1), also named BHLHA15, is a secretory cell transcription factor that belongs to the basic helix-loop-helix superfamily. MIST1 is mainly expressed in serous secreted cells and essential for the development and maturation of secretory cells. Secretory cells lacking MIST1 exhibit structural and functional defects, including alterations in cytoskeletal organization and cell polarity and improper cell-cell junction. In healthy pancreata, MIST1 facilitates cell-to-cell communication, promotes acinar cell polarity, controls Ca2+ flux, promotes unfolded protein response (UPR) homeostasis, aids in vesicle assembly, and is required for regulated exocytosis. MIST1 was downregulated in pancreatic cancer tissues compared with normal or adjacent pancreatic tissues and lower MIST1 expression in pancreatic cancer cell lines than normal acinar cells (PMID:29859299).