MIS12 Polyclonal antibody
MIS12 Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, ELISA
Conjugate
Unconjugated
Cat no : 19820-1-AP
Synonyms
Validation Data Gallery
Applications:
Tested Applications
| Positive WB detected in | HEK-293 cells, HeLa cells, HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Sample-dependent, check data in validation data gallery | |
Product Information
19820-1-AP targets MIS12 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | MIS12 fusion protein Ag13870 相同性解析による交差性が予測される生物種 |
| Full Name | MIS12, MIND kinetochore complex component, homolog (yeast) |
| Calculated molecular weight | 205 aa, 24 kDa |
| Observed molecular weight | 25-28 kDa |
| GenBank accession number | BC000229 |
| Gene symbol | MIS12 |
| Gene ID (NCBI) | 79003 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The MIS12 complex, composed of the Mis12, Pmf1, Nsl1 and Dsn1 subunits, is a highly conserved kinetochore protein that interacts with the Ndc80 and Knl1 complexes, forming the KMN network (PMID: 32341029). MIS12 is a part of the MIS12 complex which is required for normal chromosome alignment and segregation and for kinetochore formation during mitosis. MIS12 is essential for proper kinetochore microtubule attachments (PubMed:23891108)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MIS12 antibody 19820-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
