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Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using MIPEP Polyclonal antibody (11002-1-AP)

    WB analysis of HepG2 using 11002-1-AP (same clone as 11002-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 11002-1-AP (MIPEP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11002-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using MIPEP Polyclonal antibody (11002-1-AP)

    WB analysis of MCF-7 using 11002-1-AP (same clone as 11002-1-PBS)

    MCF7 cells were subjected to SDS PAGE followed by western blot with 11002-1-AP (MIPEP antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11002-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using MIPEP Polyclonal antibody (11002-1-AP)

    IHC staining of human prostate cancer using 11002-1-AP (same clone as 11002-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11002-1-AP (MIPEP antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11002-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using MIPEP Polyclonal antibody (11002-1-AP)

    IHC staining of human prostate cancer using 11002-1-AP (same clone as 11002-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer slide using 11002-1-AP (MIPEP Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 11002-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using MIPEP Polyclonal antibody (11002-1-AP)

    IHC staining of human prostate cancer using 11002-1-AP (same clone as 11002-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer slide using 11002-1-AP (MIPEP Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 11002-1-PBS in a different storage buffer formulation.

MIPEP Polyclonal antibody, PBS Only

MIPEP Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 11002-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11002-1-PBS targets MIPEP in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag1451

Product name: Recombinant human MIPEP protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-357 aa of BC009934

Sequence: MLCVGRLGGLGARAAALPPRRAGRGSLEAGIRARRVSTSWSPVGAAFNVKPQGSRLDLFGERRGLFGVPELSAPEGFHIAQEKALRKTELLVDRACSTPPGPQTVLIFDELSDSLCRVADLADFVKIAHPEPAFREAAEEACRSIGTMVEKLNTNVDLYQSLQKLLADKKLVDSLDPETRRVAELFMFDFEISGIHLDKEKRKRAVDLNVKILDLSSTFLMGTNFPNKIEKHLLPEHIRRNFTSAGDHIIIDGLHAESPDDLVREAAYKIFLYPNAGQLKCLEELLSSRDLLAKLVGYSTFSHRALQGTIAKNPETVMQFLEKLSDKLSERTLKDFEMIRGMKMKLNPQNSEVMPWD

相同性解析による交差性が予測される生物種
Full Name mitochondrial intermediate peptidase
Calculated molecular weight 81 kDa
Observed molecular weight 70-80 kDa
GenBank accession numberBC009934
Gene Symbol MIPEP
Gene ID (NCBI) 4285
RRIDAB_2235137
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ99797
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

MIPEP(Mitochondrial intermediate peptidase) is also named as MIP and belongs to the peptidase M3 family. It performs the final step in processing a specific class of nuclear-encoded proteins targeted to the mitochondrial matrix or inner membrane. The enzyme is a monomer of 75 kDa with a transit peptide and about 20% of the fmal MIP preparation consists of equimolar amounts of two peptides of 47 kDa and 28 kDa, which are apparently the products of a single cleavage of the 75 kDa protein. MIP is an independent endopeptidase acting on mitochondrial intermediate proteins as substrates.(PMID:1322290).

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