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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CCL19/MIP-3 beta Polyclonal antibody (13397-1-AP)

    WB analysis using 13397-1-AP (same clone as 13397-1-PBS)

    THP-1 cells were subjected to SDS PAGE followed by western blot with 13397-1-AP (MIP-3 Beta antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13397-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using CCL19/MIP-3 beta Polyclonal antibody (13397-1-AP)

    IF Staining of A549 using 13397-1-AP (same clone as 13397-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using MIP-3 Beta antibody (13397-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 13397-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using CCL19/MIP-3 beta Polyclonal antibody (13397-1-AP)

    IF Staining of A549 using 13397-1-AP (same clone as 13397-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol) fixed A549 cells using 13397-1-AP (MIP-3-beta antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13397-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using CCL19/MIP-3 beta Polyclonal antibody (13397-1-AP)

    IF Staining of A549 using 13397-1-AP (same clone as 13397-1-PBS)

    Immunofluorescent analysis of A549 cells using 13397-1-AP (MIP-3-beta antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13397-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of A549 cells using CCL19/MIP-3 beta Polyclonal antibody (13397-1-AP)

    FC experiment of A549 using 13397-1-AP (same clone as 13397-1-PBS)

    1X10^6 A549 cells were intracellularly stained with 0.4 ug Anti-Human MIP-3 Beta (13397-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 13397-1-PBS in a different storage buffer formulation.

CCL19/MIP-3 beta Polyclonal antibody, PBS Only

CCL19/MIP-3 beta Polyclonal Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 13397-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

CCL19, MIP-3 Beta, MIP-3-beta, Beta chemokine exodus 3, Beta-chemokine exodus-3

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

13397-1-PBS targets CCL19/MIP-3 beta in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag4200

Product name: Recombinant human MIP­3β protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-98 aa of BC027968

Sequence: MALLLALSLLVLWTSPAPTLSGTNDAEDCCLSVTQKPIPGYIVRNFHYLLIKDGCRVPAVVFTTLRGRQLCAPPDQPWVERIIQRLQRTSAKMKRRSS

相同性解析による交差性が予測される生物種
Full Name chemokine (C-C motif) ligand 19
Calculated molecular weight 98 aa, 12 kDa
Observed molecular weight11 kDa
GenBank accession numberBC027968
Gene Symbol CCL19/MIP-3 beta
Gene ID (NCBI) 6363
RRIDAB_2071529
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ99731
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

CCL19, also known as MIP-3β, is one of several CC cytokine genes clustered on the p-arm of chromosome 9. CCL19 may play a role in normal lymphocyte recirculation and homing. It also plays an important role in trafficking of T cells in thymus, and in T cell and B cell migration to secondary lymphoid organs. It specifically binds to chemokine receptor CCR7.

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