Validation Data Gallery

Filter: AllWBIPIHC

WB analysis of K-562 using 20826-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 20826-1-AP (MIOS Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of rat liver using 20826-1-AP
rat liver tissue were subjected to SDS PAGE followed by western blot with 20826-1-AP (MIOS antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 20826-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 20826-1-AP (MIOS Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of PC-3 using 20826-1-AP
PC-3 cells were subjected to SDS PAGE followed by western blot with 20826-1-AP (MIOS Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 20826-1-AP
IP result of anti-MIOS (IP:20826-1-AP, 4ug; Detection:20826-1-AP 1:300) with HeLa cells lysate 4000ug.
IHC staining of mouse skeletal muscle using 20826-1-AP
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 20826-1-AP (MIOS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse skeletal muscle using 20826-1-AP
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 20826-1-AP (MIOS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse skeletal muscle using 20826-1-AP
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 20826-1-AP (MIOS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse skeletal muscle using 20826-1-AP
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 20826-1-AP (MIOS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

MIOS Polyclonal antibody

MIOS Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 20826-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IP, ELISA

Synonyms

GATOR2 complex protein MIOS, Missing oocyte meiosis regulator homolog

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (3)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	K-562 cells, rat liver tissue, HeLa cells, PC-3 cells
Positive IP detected in	HeLa cells
Positive IHC detected in	mouse skeletal muscle tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 3 publications below

Product Information

20826-1-AP targets MIOS in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, pig
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	MIOS fusion protein Ag14802 相同性解析による交差性が予測される生物種
Full Name	missing oocyte, meiosis regulator, homolog (Drosophila)
Calculated molecular weight	875 aa, 99 kDa
Observed molecular weight	99 kDa
GenBank accession number	BC005883
Gene Symbol	MIOS
Gene ID (NCBI)	54468
RRID	AB_2878747
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9NXC5
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

The mechanistic target of rapamycin complex 1 (mTORC1) controls growth by regulating anabolic and catabolic processes in response to environmental cues, including nutrients. Amino acids signal to mTORC1 through the Rag GTPases, which are regulated by several protein complexes, including GATOR1 and GATOR2. GATOR2, which has five components (WDR24, MIOS, WDR59, SEH1L, SEC13), is required for amino acids to activate mTORC1. PMID: 35831510

Protocols

Product Specific Protocols
WB protocol for MIOS antibody 20826-1-AP	Download protocol
IHC protocol for MIOS antibody 20826-1-AP	Download protocol
IP protocol for MIOS antibody 20826-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
pig	WB	Am J Physiol Endocrinol Metab Intermittent bolus feeding does not enhance protein synthesis, myonuclear accretion, or lean growth more than continuous feeding in a premature piglet model. Authors - Marko Rudar View Article
human	WB	JCI Insight GATOR2-dependent mTORC1 activity is a therapeutic vulnerability in FOXO1 fusion positive rhabdomyosarcoma Authors - Jacqueline Morales KD Validated View Article
human	WB	Nat Commun Increased translation driven by non-canonical EZH2 creates a synthetic vulnerability in enzalutamide-resistant prostate cancer Authors - Shankha S Chatterjee View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of K-562 using 20826-1-AP

WB analysis of rat liver using 20826-1-AP

WB analysis of HeLa using 20826-1-AP

WB analysis of PC-3 using 20826-1-AP

IP experiment of HeLa using 20826-1-AP

IHC staining of mouse skeletal muscle using 20826-1-AP

IHC staining of mouse skeletal muscle using 20826-1-AP

IHC staining of mouse skeletal muscle using 20826-1-AP

IHC staining of mouse skeletal muscle using 20826-1-AP

MIOS Polyclonal antibody

MIOS Polyclonal Antibody for WB, IHC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Intermittent bolus feeding does not enhance protein synthesis, myonuclear accretion, or lean growth more than continuous feeding in a premature piglet model.

GATOR2-dependent mTORC1 activity is a therapeutic vulnerability in FOXO1 fusion positive rhabdomyosarcoma

Increased translation driven by non-canonical EZH2 creates a synthetic vulnerability in enzalutamide-resistant prostate cancer