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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MINK1 Polyclonal antibody

MINK1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 13137-1-AP

Synonyms

B55, GCK family kinase MiNK, hMINK, hMINKbeta, MAP4K6, MEK kinase kinase 6, MEKKK 6, MINK, MINK1, Misshapen like kinase 1, Misshapen/NIK related kinase, YSK2, ZC3

Validation Data Gallery

Applications:
  • WB analysis using 13137-1-AP

    WB analysis using 13137-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13137-1-AP (MINK1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse brain using 13137-1-AP

    WB analysis of mouse brain using 13137-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 13137-1-AP (MINK1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse brain using 13137-1-AP

    IP experiment of mouse brain using 13137-1-AP

    IP result of anti-MINK1 (IP:13137-1-AP, 4ug; Detection:13137-1-AP 1:300) with mouse brain tissue lysate 4000ug.

  • IHC staining of human lymphoma using 13137-1-AP

    IHC staining of human lymphoma using 13137-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 13137-1-AP (MINK1 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human lymphoma using 13137-1-AP

    IHC staining of human lymphoma using 13137-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 13137-1-AP (MINK1 Antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human skeletal muscle using 13137-1-AP

    IHC staining of human skeletal muscle using 13137-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 13137-1-AP (MINK1 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human skeletal muscle using 13137-1-AP

    IHC staining of human skeletal muscle using 13137-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 13137-1-AP (MINK1 Antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of mouse brain using 13137-1-AP

    IHC staining of mouse brain using 13137-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13137-1-AP (MINK1 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of mouse brain using 13137-1-AP

    IHC staining of mouse brain using 13137-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13137-1-AP (MINK1 Antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman lymphoma tissue, human skeletal muscle tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

13137-1-AP targets MINK1 in WB, IP, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MINK1 fusion protein Ag3795 相同性解析による交差性が予測される生物種
Full Name misshapen-like kinase 1 (zebrafish)
Calculated molecular weight 1332 aa, 150 kDa
Observed molecular weight 150 kDa
GenBank accession numberBC034673
Gene symbol MINK1
Gene ID (NCBI) 50488
RRIDAB_10644151
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for MINK1 antibody 13137-1-APDownload protocol
IHC protocol for MINK1 antibody 13137-1-APDownload protocol
IP protocol for MINK1 antibody 13137-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,CoIP

Hum Mol Genet

Lung adenocarcinoma cell-derived exosomes promote M2 macrophage polarization through transmission of miR-3153 to activate the JNK signaling pathway

Authors - Lu Xu
View Article
ratWB

Cardiovasc Ther

Effects of Vitamin D Deficiency on the Function of the Cardiac Autonomic Nervous System in Rats.

Authors - Xuemei Luo
View Article
mouseIHC

Front Genet

MBD2 mediates Th17 cell differentiation by regulating MINK1 in Th17-dominant asthma

Authors - Zhifeng Chen
View Article