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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MIF Recombinant monoclonal antibody (83199-2-RR)

    WB analysis using 83199-2-RR (same clone as 83199-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83199-2-RR (MIF antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83199-2-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of RAW 264.7 cells using MIF Recombinant monoclonal antibody (83199-2-RR)

    FC experiment of RAW 264.7 using 83199-2-RR (same clone as 83199-2-PBS)

    1x10^6 RAW 264.7 cells were intracellularly stained with 0.25 ug MIF Recombinant antibody (83199-2-RR, Clone:230529F2) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 83199-2-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of Neuro-2a cells using MIF Recombinant monoclonal antibody (83199-2-RR)

    FC experiment of Neuro-2a using 83199-2-RR (same clone as 83199-2-PBS)

    1x10^6 Neuro-2a cells were intracellularly stained with 0.25 ug MIF Recombinant antibody (83199-2-RR, Clone:230529F2) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 83199-2-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of NIH/3T3 cells using MIF Recombinant monoclonal antibody (83199-2-RR)

    FC experiment of NIH/3T3 using 83199-2-RR (same clone as 83199-2-PBS)

    1x10^6 NIH/3T3 cells were intracellularly stained with 0.2 ug MIF Recombinant antibody (83199-2-RR, Clone:230529F2) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.2 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 83199-2-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83199-2-PBS

    Affinity and Kinetic Characterization of 83199-2-PBS

    Biolayer interferometry (BLl) kinetic assay of 83199-2-PBS against Mouse MIF was performed. The affinity constant is 0.473 nM.

  • Affinity and Kinetic Characterization of 83199-2-RR

    Affinity and Kinetic Characterization of 83199-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83199-2-RR against Mouse MIF were performed. The affinity constant is 0.131 nM.

Mouse MIF Recombinant monoclonal antibody, PBS Only

MIF Uni-rAb® Recombinant Antibody for WB, FC (Intra), Indirect ELISA
Cat No. 83199-2-PBS
Clone No.230529F2

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), Indirect ELISA

Synonyms

230529F2, GIF

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83199-2-PBS targets MIF in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Eg0574

Product name: Recombinant mouse MIF protein

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: C-6*HIS

Domain: 2-115 aa of NM_010798.2

Sequence: PMFIVNTNVPRASVPEGFLSELTQQLAQATGKPAQYIAVHVVPDQLMTFSGTNDPCALCSLHSIGKIGGAQNRNYSKLLCGLLSDRLHISPDRVYINYYDMNAANVGWNGSTFA

相同性解析による交差性が予測される生物種
Full Name macrophage migration inhibitory factor
Calculated molecular weight13KD
Observed molecular weight13 kDa
GenBank accession numberNM_010798.2
Gene Symbol Mif
Gene ID (NCBI) 17319
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP34884
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Macrophage migration inhibitory factor (MIF) is a homo-trimeric protein that acts as a pleiotropic pro-inflammatory cytokine. It is involved in various functions, including leukocyte recruitment, inflammation, immune responses, cell proliferation, tumorigenesis, and counter-regulation of glucocorticoids (GC). MIF is a highly conserved protein of 12.5 kDa, with evolutionarily ancient homologues in plants, protozoans, nematodes, and invertebrates.

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