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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse uterus tissue using MIER3 Polyclonal antibody (17543-1-AP)

    WB analysis of mouse uterus using 17543-1-AP (same clone as 17543-1-PBS)

    mouse uterus tissue were subjected to SDS PAGE followed by western blot with 17543-1-AP (MIER3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17543-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse uterus tissue using MIER3 Polyclonal antibody (17543-1-AP)

    WB analysis of mouse uterus using 17543-1-AP (same clone as 17543-1-PBS)

    mouse uterus tissue were subjected to SDS PAGE followed by western blot with 17543-1-AP (MIER3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17543-1-PBS in a different storage buffer formulation.

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MIER3 Polyclonal antibody, PBS Only

MIER3 Polyclonal Antibody for WB, Indirect ELISA
Cat No. 17543-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

17543-1-PBS targets MIER3 in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MIER3 fusion protein Ag11604 相同性解析による交差性が予測される生物種
Full Name mesoderm induction early response 1, family member 3
Calculated molecular weight 522 aa, 59 kDa
Observed molecular weight 55 kDa
GenBank accession numberBC041348
Gene Symbol MIER3
Gene ID (NCBI) 166968
RRIDAB_2143938
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ7Z3K6
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

The mesoderm induction early response (MIER) protein family is known as fibroblast growth factor (FGF)-regulated immediate-early protein family. Activated by FGF, MIER proteins may have a pivotal role in FGF-regulated cellular activities, indicating a potential influence in the progression of certain cancers [PMID:15117948]. MIER3 localizes to the nucleus and primarily functions as a transcriptional repressor. Rat mammary carcinoma susceptibility 1b (Mcs1b) is a quantitative trait locus on RN02 that confers decreased susceptibility when Copenhagen (COP)-resistant alleles are introgressed into a Wistar Furth (WF)-susceptible genome. MIER3 is a candidate Mcs1b gene [PMID:22993404].