Validation Data Gallery

Filter: AllWBIHCFC (Intra)

WB analysis of human testis using 11452-1-AP
human testis tissue were subjected to SDS PAGE followed by western blot with 11452-1-AP (MIER1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 11452-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 11452-1-AP (MIER1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 11452-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 11452-1-AP (MIER1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse heart using 11452-1-AP
mouse heart tissue were subjected to SDS PAGE followed by western blot with 11452-1-AP (MIER1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IHC staining of human pancreas cancer using 11452-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11452-1-AP (MIER1 Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human pancreas cancer using 11452-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 11452-1-AP (MIER1 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human pancreas cancer using 11452-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11452-1-AP (MIER1 Antibody) at dilution of 1:50 (under 10x lens).
FC experiment of A431 using 11452-1-AP
1x10^6 A431 cells were intracellularly stained with 0.25 ug MIER1 Polyclonal antibody (11452-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

MIER1 Polyclonal antibody

MIER1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 11452-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ChIP, ELISA

Synonyms

MIER 1, MI ER1, hMi-er1, hMIER1, hMI ER1

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (3)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	human testis tissue, HepG2 cells, mouse heart tissue
Positive IHC detected in	human pancreas cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive FC (Intra) detected in	A431 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 2 publications below
IHC	See 1 publications below
ChIP	See 1 publications below

Product Information

11452-1-AP targets MIER1 in WB, IHC, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	MIER1 fusion protein Ag2010 相同性解析による交差性が予測される生物種
Full Name	mesoderm induction early response 1 homolog (Xenopus laevis)
Calculated molecular weight	536 aa, 61 kDa
Observed molecular weight	82 kDa
GenBank accession number	BC017423
Gene Symbol	MIER1
Gene ID (NCBI)	57708
RRID	AB_10733247
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q8N108
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for MIER1 antibody 11452-1-AP	Download protocol
IHC protocol for MIER1 antibody 11452-1-AP	Download protocol
FC protocol for MIER1 antibody 11452-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,ChIP	Gastroenterology Proteomic characterization identifies clinically relevant subgroups of gastrointestinal stromal tumors Authors - Mingjun Sun View Article
mouse	IHC	Nat Commun Acute liver steatosis translationally controls the epigenetic regulator MIER1 to promote liver regeneration in a study with male mice Authors - Yanhao Chen View Article
human	WB	Cell Chem Biol HDAC3 and HDAC8 PROTAC dual degrader reveals roles of histone acetylation in gene regulation Authors - Yufeng Xiao View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of human testis using 11452-1-AP

WB analysis of HepG2 using 11452-1-AP

WB analysis of HepG2 using 11452-1-AP

WB analysis of mouse heart using 11452-1-AP

IHC staining of human pancreas cancer using 11452-1-AP

IHC staining of human pancreas cancer using 11452-1-AP

IHC staining of human pancreas cancer using 11452-1-AP

FC experiment of A431 using 11452-1-AP

MIER1 Polyclonal antibody

MIER1 Polyclonal Antibody for WB, IHC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Proteomic characterization identifies clinically relevant subgroups of gastrointestinal stromal tumors

Acute liver steatosis translationally controls the epigenetic regulator MIER1 to promote liver regeneration in a study with male mice

HDAC3 and HDAC8 PROTAC dual degrader reveals roles of histone acetylation in gene regulation