Validation Data Gallery
Tested Applications
| Positive WB detected in | T-47D cells, BT-474 cells, MDA-MB-231 cells, DU 145 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
87911-1-RR targets MIEN1 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Eg5748 Product name: Recombinant Human MIEN1 protein (rFc Tag) Source: mammalian cells-derived, V37 Tag: C-rFc Domain: 2-115 aa of NM_032339.5 Sequence: SGEPGQTSVAPPPEEVEPGSGVRIVVEYCEPCGFEATYLELASAVKEQYPGIEIESRLGGTGAFEIEINGQLVFSKLENGGFPYEKDLIEAIRRASNGETLEKITNSRPPCVIL 相同性解析による交差性が予測される生物種 |
| Full Name | chromosome 17 open reading frame 37 |
| Calculated molecular weight | 12 kDa |
| Observed molecular weight | 12 kDa |
| GenBank accession number | NM_032339.5 |
| Gene Symbol | C17orf37 |
| Gene ID (NCBI) | 84299 |
| RRID | AB_3745822 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9BRT3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
MIEN1 (Migration and Invasion Enhancer 1), also known as C17orf37 or RDEP, is a protein-coding gene located in the q12 region of chromosome 17. Through prenylation modification, this protein anchors to the inner side of the cell membrane, promotes actin cytoskeleton rearrangement and membrane ruffling, thereby enhancing the migration and invasion capabilities of tumor cells. MIEN1 is highly expressed in various malignant tumors, including breast cancer, prostate cancer, and lung cancer, and its expression level is closely correlated with tumor metastasis, clinical stage, and poor patient prognosis. Moreover, MIEN1 promotes epithelial-mesenchymal transition (EMT) by regulating signaling pathways such as PI3K/AKT and NF-κB, making it a potential anti-metastatic therapeutic target and prognostic marker.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MIEN1 antibody 87911-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |