Validation Data Gallery
Tested Applications
| Positive WB detected in | A549 cells, Calu-1 cells, HepG2 cells, L02 cells, MG-63 cells, U2OS cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
33503-1-AP targets MICALL1 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag39169 Product name: Recombinant human MICALL1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 750-863 aa of NM_033386.3 Sequence: NLEQRQADVEYELRCLLNKPEKDWTEEDRAREKVLMQELVTLIEQRNAIINCLDEDRQREEEEDKMLEAMIKKKEFQREAEPEGKKKGKFKTMKMLKLLGNKRDAKSKSPRDKS 相同性解析による交差性が予測される生物種 |
| Full Name | MICAL-like 1 |
| Calculated molecular weight | 93kDa，863aa |
| Observed molecular weight | 125 kDa |
| GenBank accession number | NM_033386.3 |
| Gene Symbol | MICALL1 |
| Gene ID (NCBI) | 85377 |
| RRID | AB_3742994 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q8N3F8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
MICALL1 (MICAL-like protein 1) is a protein involved in the regulation of cytoskeleton dynamics and is a member of the MICAL family. This protein interacts with other molecules through its N-terminal CH domain and C-terminal coiled-coil domain, primarily regulating the stability of microtubules and actin, thereby affecting processes such as cell shape, migration, and intracellular transport. Studies have shown that MICALL1 plays a key role in neuronal axon guidance, angiogenesis, and the establishment of epithelial cell polarity. Additionally, it binds to Rab GTPases (such as Rab8a) and is involved in vesicle transport and membrane remodeling. Aberrant expression of MICALL1 is associated with tumor metastasis and neurological diseases, with its molecular mechanisms involving precise regulation of cytoskeleton rearrangement, making it a potential therapeutic target.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MICALL1 antibody 33503-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |