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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MICAL2 Polyclonal antibody (13965-1-AP)

    WB analysis using 13965-1-AP (same clone as 13965-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 13965-1-AP (MICAL2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13965-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of PC-3 cells using MICAL2 Polyclonal antibody (13965-1-AP)

    WB analysis of PC-3 using 13965-1-AP (same clone as 13965-1-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 13965-1-AP (MICAL2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13965-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using MICAL2 Polyclonal antibody (13965-1-AP)

    IHC staining of human prostate cancer using 13965-1-AP (same clone as 13965-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13965-1-AP (MICAL2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13965-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using MICAL2 Polyclonal antibody (13965-1-AP)

    IHC staining of human prostate cancer using 13965-1-AP (same clone as 13965-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13965-1-AP (MICAL2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13965-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using MICAL2 Polyclonal antibody (13965-1-AP)

    IHC staining of human prostate cancer using 13965-1-AP (same clone as 13965-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13965-1-AP (MICAL2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13965-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using MICAL2 Polyclonal antibody (13965-1-AP)

    IF Staining of HepG2 using 13965-1-AP (same clone as 13965-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MICAL2 antibody (13965-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 13965-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using MICAL2 Polyclonal antibody (13965-1-AP)

    IF Staining of HepG2 using 13965-1-AP (same clone as 13965-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using MICAL2 antibody (13965-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13965-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

MICAL2 Polyclonal antibody, PBS Only

MICAL2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 13965-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

13965-1-PBS targets MICAL2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MICAL2 fusion protein Ag4950 相同性解析による交差性が予測される生物種
Full Name microtubule associated monoxygenase, calponin and LIM domain containing 2
Calculated molecular weight 127 kDa
Observed molecular weight 95 kDa, 112 kDa
GenBank accession numberBC044577
Gene Symbol MICAL2
Gene ID (NCBI) 9645
RRIDAB_2143764
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO94851
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

MICALs (Molecules Interacting with CasL) are atypical multidomain flavoenzymes with diverse cellular functions. There are three known isoforms, MICAL1, MICAL2, and MICAL3, as well as the MICAL-like proteins MICAL-L1 and MICAL-L2. MICAL2 has three conserved domains: an N-terminal flavin adenine dinucleotide (FAD) binding domain, a calponin homology (CH) domain, and a Lin11, Isl-1, and Mec-3 (LIM) domain. It has been demonstrated that MICAL2 could regulate actin stress fibers and is required for normal actin organization. In addition, MICAL2-PV, a novel splicing variant of MICAL2, has been reported to be involved in cancer progression of prostate cancer. This antibody can recognize both MICAL2 and MICAL2-PV. This antibody recognizes various isoforms of MICAL2 around 90-95 kDa or 109-112 kDa.