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  • KD/KO Validated

MICAL1 Polyclonal antibody

MICAL1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 14818-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

[F-actin]-monooxygenase MICAL1, EC:1.14.13.225, MICAL, NEDD9-interacting protein with calponin homology and LIM domains, NICAL

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -


ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inJurkat cells, HEK-293 cells, HeLa cells, human brain tissue, T-47D cells
Positive IP detected inHeLa cells, T-47D cells
Positive IHC detected inhuman lung tissue, human heart tissue, human ovary tissue, human placenta tissue, human skin tissue, human spleen tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14818-1-AP targets MICAL1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MICAL1 fusion protein Ag6578 相同性解析による交差性が予測される生物種
Full Name microtubule associated monoxygenase, calponin and LIM domain containing 1
Calculated molecular weight 118 kDa
Observed molecular weight 120 kDa
GenBank accession numberBC052983
Gene Symbol MICAL1
Gene ID (NCBI) 64780
RRIDAB_2143754
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8TDZ2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MICALs (Molecules Interacting with CasL) are atypical multidomain flavoenzymes with diverse cellular functions.There are three known isoforms, MICAL1, MICAL2 and MICAL3, as well as the MICAL-like proteins MICAL-L1 and MICAL-L2. MICAL1 has four conserved domains: an N-terminal flavin adenine dinucleotide (FAD) binding domain, a calponin homology (CH) domain, a Lin11, Isl-1 and Mec-3 (LIM) domain and a C-terminal coiled-coil (CC) domain. MICAL1 is reported to regulate actin stress fibers and be required for normal actin organization. It may also be involved in apoptosis through binding with NDR (nuclear Dbf2-related) kinases. This antibody specially recognizes MICAL1.

Protocols

Product Specific Protocols
WB protocol for MICAL1 antibody 14818-1-APDownload protocol
IHC protocol for MICAL1 antibody 14818-1-APDownload protocol
IF protocol for MICAL1 antibody 14818-1-APDownload protocol
IP protocol for MICAL1 antibody 14818-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Sci Adv

F-actin disassembly factor MICAL1 binding to Myosin Va mediates cargo unloading during cytokinesis.

Authors - Fengfeng Niu
  • KO Validated
humanWB

Nat Commun

Oxidation of F-actin controls the terminal steps of cytokinesis.

Authors - Stéphane Frémont
  • KD Validated
humanCoIP

Dev Cell

Amplification of F-Actin Disassembly and Cellular Repulsion by Growth Factor Signaling.

Authors - Jimok Yoon
humanWB

J Cell Biol

MICAL2 enhances branched actin network disassembly by oxidizing Arp3B-containing Arp2/3 complexes.

Authors - Chiara Galloni
  • KD Validated
humanIHC

Oncogene

Phosphorylation of MICAL2 by ARG promotes head and neck cancer tumorigenesis by regulating skeletal rearrangement

Authors - Ze Zhang

Diabetes

Semaphorin3a promotes advanced diabetic nephropathy.

Authors - Pardeep K Aggarwal
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