Validation Data Gallery
Tested Applications
| Positive WB detected in | HepG2 cells, HuH-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
31986-1-AP targets CTAGE5 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag34060 Product name: Recombinant human MIA2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 32-376 aa of BC130537 Sequence: CGDLECEALINRVSAMRDYRGPDCRYLNFTKGEEISVYVKLAGEREDLWAGSKGKEFGYFPRDAVQIEEVFISEEIQMSTKESDFLCLLGVSYTFDNEDSELNGDYGENIYPYEEDKDEKSSIYESDFQIEPGFYATYESTLFEDQVPALEAPEDIGSTSESKDWEEVVVESMEQDRIPEVHVPPSSAVSGVKEWFGLGGEQAEEKAFESVIEPVQESSFRSRKIAVEDENDLEELNNGEPQTEHQQESESEIDSVPKTQSELASESEHIPKPQSTGWFGGGFTSYLGFGDEDTGLELIAEESNPPLQDFPNSISSDKEATVPCTEILTEKKDTITNDSLSLKPS 相同性解析による交差性が予測される生物種 |
| Full Name | CTAGE family, member 5 |
| Calculated molecular weight | 160 kDa |
| Observed molecular weight | 240 kDa |
| GenBank accession number | BC130537 |
| Gene Symbol | CTAGE5 |
| Gene ID (NCBI) | 4253 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q96PC5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Cutaneous T-cell lymphoma-associated antigen 5 (CTAGE5, also known as MIA2) is a protein involved in the regulation of intracellular transport and secretion. It plays a crucial role in the trafficking of large cargo proteins, such as collagen VII, from the endoplasmic reticulum (ER) to the Golgi apparatus (PMID: 25202031; 27170179). CTAGE5 forms a complex with TANGO1, another cargo receptor, to facilitate the export of collagen VII from the ER (PMID: 21525241). This process is essential for the proper secretion of collagen and other large proteins, which are critical for maintaining tissue integrity and function. Additionally, CTAGE5 produces 240 and 120 kDa protein isoforms (PMID: 21807889).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CTAGE5 antibody 31986-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |