Validation Data Gallery
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Tested Applications
| Positive IHC detected in | human skin cancer tissue, human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| IHC | See 1 publications below |
Product Information
15734-1-AP targets MIA in IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag8499 Product name: Recombinant human MIA protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 24-131 aa of BC005910 Sequence: GGPMPKLADRKLCADQECSHPISMAVALQDYMAPDCRFLTIHRGQVVYVFSKLKGRGRLFWGGSVQGDYYGDLAARLGYFPSSIVREDQTLKPGKVDVKTDKWDFYCQ 相同性解析による交差性が予測される生物種 |
| Full Name | melanoma inhibitory activity |
| Calculated molecular weight | 131 aa, 15 kDa |
| GenBank accession number | BC005910 |
| Gene Symbol | MIA |
| Gene ID (NCBI) | 8190 |
| RRID | AB_2878176 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q16674 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Melanoma-derived growth regulatory protein, also known as MIA, CD RAP, is a protein that in humans is encoded by the MIA gene. It is a marker for malignant melanoma (PMID: 9242442).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for MIA antibody 15734-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |