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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MGST2 Polyclonal antibody (25451-1-AP)

    WB analysis using 25451-1-AP (same clone as 25451-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 25451-1-AP (MGST2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25451-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HUVEC cells using MGST2 Polyclonal antibody (25451-1-AP)

    IP experiment of HUVEC using 25451-1-AP (same clone as 25451-1-PBS)

    IP result of anti-MGST2 (IP:25451-1-AP, 4ug; Detection:25451-1-AP 1:400) with HUVEC cells lysate 1040 ug. This data was developed using the same antibody clone with 25451-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HuH-7 cells using MGST2 Polyclonal antibody (25451-1-AP)

    IP experiment of HuH-7 using 25451-1-AP (same clone as 25451-1-PBS)

    IP result of anti-MGST2 (IP:25451-1-AP, 4ug; Detection:25451-1-AP 1:400) with HuH-7 cells lysate 1040 ug. This data was developed using the same antibody clone with 25451-1-PBS in a different storage buffer formulation.

MGST2 Polyclonal antibody, PBS Only

MGST2 Polyclonal Antibody for WB, IP, Indirect ELISA
Cat No. 25451-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, Indirect ELISA

Synonyms

GST2, MGST II, Microsomal GST 2, Microsomal GST II

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

25451-1-PBS targets MGST2 in WB, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag21105

Product name: Recombinant human MGST2 protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 1-147 aa of BC025416

Sequence: MAGNSILLAAVSILSACQQSYFALQVGKARLKYKVTPPAVTGSPEFERVFRAQQNCVEFYPIFIITLWMAGWYFNQVFATCLGLVYIYGRHLYFWGYSEAAKKRITGFRLSLGILALLTLLGALGIANSFLDEYLDLNIAKKLRRQF

相同性解析による交差性が予測される生物種
Full Name microsomal glutathione S-transferase 2
Calculated molecular weight 147 aa, 17 kDa
Observed molecular weight17 kDa
GenBank accession numberBC025416
Gene Symbol MGST2
Gene ID (NCBI) 4258
RRIDAB_3669476
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ99735
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Microsomal glutathione S-transferase 2 (MGST2) is a 17 kDa trimeric integral membrane protein homologous to leukotriene C4 synthase (LTC4S) and catalyze the biosynthesis of the proinflammatory mediator leukotriene C4 (LTC4) in cells devoid of LTC4S (PMID: 23409838).

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