Validation Data Gallery

Filter: AllWBIHC

WB analysis using 11285-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11285-1-AP (RNF156 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 11285-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 11285-1-AP (RNF156 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 11285-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 11285-1-AP (RNF156 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 11285-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 11285-1-AP (RNF156 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 11285-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 11285-1-AP (RNF156 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 11285-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 11285-1-AP (RNF156 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 11285-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 11285-1-AP (RNF156 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC staining of human lung cancer using 11285-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11285-1-AP (RNF156 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 11285-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11285-1-AP (RNF156 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 11285-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer using 11285-1-AP (RNF156 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human lung cancer using 11285-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer using 11285-1-AP (RNF156 antibody) at dilution of 1:50 (under 40x lens).

Featured Product
KD/KO Validated

RNF156 Polyclonal antibody

RNF156 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 11285-1-AP

Publications(10)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Synonyms

KIAA0544, mahogunin, ring finger 1, MGRN1, RING finger protein 156, RNF156

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (10)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HEK-293 cells, HeLa cells, HepG2 cells, mouse brain tissue
Positive IHC detected in	human lung cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:3000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 8 publications below
IHC	See 1 publications below
IF	See 3 publications below
IP	See 1 publications below

Product Information

11285-1-AP targets RNF156 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag1808 Product name: Recombinant human MGRN1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-310 aa of BC014199 Sequence: SDDENSDNSNECVVCLSDLRDTLILPCRHLCLCTSCADTLRYQANNCPICRLPFRALLQIRAVRKKPGALSPVSFSPVLAQSLEHDEHSCPFKKSKPHPASLASKKPKRETNSDSVPPGYEPISLLEALNGLRAVSPAIPSAPLYEEITYSGISDGLSQASCPLAAIDHILDSSRQKGRPQSKAPDSTLRSPSSPIHEEDEEKLSEDVDAPPPLGGAELALRESSSPESFITEEVDESSSPQQGTRAASIENVLQDSSPEHCGRGPPADIYLPGRPTSMETAHGLATTSPTWPPLGGPSPDPSAAELTPL 相同性解析による交差性が予測される生物種
Full Name	mahogunin, ring finger 1
Calculated molecular weight	63 kDa
Observed molecular weight	58-64 kDa
GenBank accession number	BC014199
Gene Symbol	MGRN1
Gene ID (NCBI)	23295
RRID	AB_2143351
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	O60291
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

MGRN1(E3 ubiquitin-protein ligase) mediates monoubiquitination at multiple sites of TSG101 in the presence of UBE2D1, but not of UBE2G1, nor UBE2H. It also plays a role in the regulation of endosome-to-lysosome trafficking. Human and mouse mahogunin exhibit a similar expression pattern across multiple tissues with particularly high levels in brain(PMID: 12560552). It has 4 isoforms produced by alternative splicing with the molecular mass of 58-64 kDa.

Protocols

Product Specific Protocols
IHC protocol for RNF156 antibody 11285-1-AP	Download protocol
WB protocol for RNF156 antibody 11285-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,IF	Mol Cell Aging Triggers Cytoplasmic Depletion and Nuclear Translocation of the E3 Ligase Mahogunin: A Function for Ubiquitin in Neuronal Survival. Authors - Stefano Benvegnù KD Validated View Article
human	WB	Dev Cell A Membrane-Tethered Ubiquitination Pathway Regulates Hedgehog Signaling and Heart Development. Authors - Jennifer H Kong View Article
human	WB	Pigment Cell Melanoma Res MGRN1-dependent pigment-type switching requires its ubiquitination activity but not its interaction with TSG101 or NEDD4. Authors - Gunn Teresa M TM View Article
human,mouse	IP	Development Gene-teratogen interactions influence the penetrance of birth defects by altering Hedgehog signaling strength. Authors - Jennifer H Kong View Article
human	WB,IHC	Front Oncol Promoter Methylation of the MGRN1 Gene Predicts Prognosis and Response to Chemotherapy of High-Grade Serous Ovarian Cancer Patients. Authors - Xiao-Fei Li View Article
mouse	WB,IF	J Biol Chem Mahoganoid and mahogany mutations rectify the obesity of the yellow mouse by effects on endosomal traffic of MC4R protein. Authors - Overton John D JD KD Validated View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 11285-1-AP

WB analysis of mouse brain using 11285-1-AP

WB analysis of mouse brain using 11285-1-AP

WB analysis of HeLa using 11285-1-AP

WB analysis of HepG2 using 11285-1-AP

WB analysis of HEK-293 using 11285-1-AP

WB analysis of mouse brain using 11285-1-AP

IHC staining of human lung cancer using 11285-1-AP

IHC staining of human lung cancer using 11285-1-AP

IHC staining of human lung cancer using 11285-1-AP

IHC staining of human lung cancer using 11285-1-AP

RNF156 Polyclonal antibody

RNF156 Polyclonal Antibody for WB, IHC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Aging Triggers Cytoplasmic Depletion and Nuclear Translocation of the E3 Ligase Mahogunin: A Function for Ubiquitin in Neuronal Survival.

A Membrane-Tethered Ubiquitination Pathway Regulates Hedgehog Signaling and Heart Development.

MGRN1-dependent pigment-type switching requires its ubiquitination activity but not its interaction with TSG101 or NEDD4.

Gene-teratogen interactions influence the penetrance of birth defects by altering Hedgehog signaling strength.

Promoter Methylation of the MGRN1 Gene Predicts Prognosis and Response to Chemotherapy of High-Grade Serous Ovarian Cancer Patients.

Mahoganoid and mahogany mutations rectify the obesity of the yellow mouse by effects on endosomal traffic of MC4R protein.